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Method for identifying Pingli fiveleaf gynostemma herb through near infrared spectroscopy

A technology of near-infrared spectroscopy and Gynostemma pentaphyllum, applied in the field of near-infrared spectroscopy detection and analysis

Inactive Publication Date: 2014-05-07
HEBEI UNIVERSITY
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Problems solved by technology

These reports confirm that there are differences in the composition of Gynostemma from different origins, so these methods have certain reference value for identifying the authenticity of Gynostemma from different origins, but there is no report on the authenticity of Gynostemma pingli by using its composition differences

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  • Method for identifying Pingli fiveleaf gynostemma herb through near infrared spectroscopy
  • Method for identifying Pingli fiveleaf gynostemma herb through near infrared spectroscopy
  • Method for identifying Pingli fiveleaf gynostemma herb through near infrared spectroscopy

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Embodiment Construction

[0051] The present invention will be further described in detail below in conjunction with the accompanying drawings and specific embodiments.

[0052] 1. Instruments and reagents: The MPA near-infrared spectrometer and diffuse reflectance accessories from Bruker, Germany, are used to collect sample spectra. The instrument comes with analysis software and MATLAB software.

[0053] 1-1. Instrument noise

[0054] Configure a regulated power supply, turn on the machine and preheat until the instrument is fully stable to ensure a suitable test environment temperature of 15-25°C;

[0055] 1-2. Wavelength accuracy and reproducibility

[0056] Use a low-pressure mercury lamp and a methylene blue solution with a mass fraction of 0.005% to calibrate the accuracy of the wavelength to prevent drift.

[0057] 2. Sample preparation and spectral scanning: Gynostemma pentaphylla samples were purchased at the place of origin.

[0058] Table 1 Gynostemma pentaphyllum sample information table ...

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Abstract

The invention provides a method for identifying Pingli fiveleaf gynostemma herb through near infrared spectroscopy, which comprises the following steps: (A) establishing a near infrared spectroscopy identifying model of Pingli fiveleaf gynostemma herb: (A-1) selecting the spectral range of 4,000-12,500cm<-1>, and scanning the near infrared spectrogram of the Pingli fiveleaf gynostemma herb; (A-2) pretreating the data of the spectral range of 4,000-9,500cm<-1>; (A-3) extracting the main components; and (A-4) establishing an artificial neutral network model: determining the structure of the neutral network by an artificial neutral network algorithm according to the characteristics of the input and output data, and training the neutral network by use of the training data; and establishing a BP artificial neutral network model of input layer nodes 10-, hidden layer nodes 5- and output layer nodes 2 by use of the MATLAB software; and (B) identifying the unknown sample: scanning the near infrared spectrogram of the unknown sample under the same conditions, selecting the number of main components, judging the authenticity of the unknown sample according to the trained neutral network model, and representing the output nodes with binary codes respectively, wherein 10 represents Pingli fiveleaf gynostemma herb, and 01 represents non-Pingli fiveleaf gynostemma herb.

Description

technical field [0001] The invention relates to a method for discriminating Gynostemma pycnogenol by near-infrared spectrum, in particular to a method for discriminating Gynostemma pycnogenol by using near-infrared spectrum technology combined with an artificial neural network algorithm, and belongs to the field of detection and analysis by near-infrared spectrum. Background technique [0002] Gynostemma pentaphyllum, also known as Aescin and Aescin, has the functions of lowering blood pressure, lowering blood fat, lowering blood sugar, protecting the heart and liver, regulating fat and losing weight, and has the title of "herb for longevity and longevity". Since the General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China implemented the geographical protection of the origin of Shaanxi Pingli Jiaogulan in 2004, the value of Pingli Jiaogulan has doubled, and the phenomenon of shoddy, fake and shoddy products has occurred from ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/359
Inventor 赵志磊李小亭陈培云吴广臣刘秀华
Owner HEBEI UNIVERSITY
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