Device and method for detecting pulsating pressure of tissue engineering myocardial cell
A cardiomyocyte and tissue engineering technology, applied in the field of biomedical engineering, can solve problems such as unsatisfactory effect, mismatching of the mechanical properties of cardiomyocytes with host cells, and no measurement of cardiac muscle cell pulsatile pressure, achieving simple operation and good applicability. Effect
Active Publication Date: 2014-05-21
HANGZHOU BAIQIAO MEDICAL TECH CO LTD
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Problems solved by technology
[0002] Cell therapy has broad prospects for the treatment of myocardial infarction and end-stage heart disease, but the current clinical effect is not very satisfactory. One of the main reasons is that the mechanical properties of cardiomyocytes cultured in vitro do not match those of host cells. A device for the mechanical properties of cardiomyocytes, which is of great significance for the application of cell therapy in clinical medicine
[0003] The most important function of cardiomyocytes is pulsation, that is, regular contraction movement. Therefore, the most important thing to measure the mechanical properties of cardiomyocytes is to measure their pulsating pressure - systolic pressure and diastolic pressure, but there is no measurement of cardiomyocyte pulsating pressure. device
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[0020] The present invention will be further described below in conjunction with accompanying drawing.
[0021] like figure 1 As shown, a device for measuring the pulsation pressure of cardiomyocytes mainly consists of three parts: a culture chamber unit, a measurement unit, and a micro-pressure adjustment unit.
[0022] The culture room unit includes a sealing plug 1; a culture solution outlet 2; a clean air inlet (culture solution inlet) 22; a culture observation room cover plate 23; a cardiomyocyte culture room 24;
[0023] The cover plate 23 of the culture observation chamber is connected with the cardiomyocyte culture chamber 24 through the fifth fixing screw 25 . There is a culture solution outlet 2 on the left side of the cardiomyocyte culture chamber 24, and a sealing plug 1 is arranged at the outlet. There is a clean air inlet (culture solution inlet) 22 on the right side of the cardiomyocyte culture chamber 24 . The role of the culture room is to cultivate the...
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The invention relates to a device and a method for detecting the pulsating pressure of a tissue engineering myocardial cell. According to the device, the size of the pressure intensity of a myocardial cell culture room can be regulated by respectively injecting and discharging clean air in and out of the myocardial cell culture room, and the size of the pulsating pressure of the myocardial cell is measured by observing pulsating conditions of the myocardial cell by virtue of a microscope at the different pressure intensities; in the measurement process, the pressure intensity is increased, when an operator observes that the myocardial cell in the culture room stops beating suddenly, a value displayed by a pressure meter is a systolic pressure, then the pressure intensity is decreased until reaching a moment when the operator observes that the myocardial cell beats again, and the value displayed by the pressure meter at the moment is a diastolic pressure. As the device is provided with high-precision measuring instruments and a micro-pressure regulating device, the measured data is accurate and reliable.
Description
technical field [0001] The invention belongs to the field of biomedical engineering, and relates to a detection device for tissue engineered cardiomyocyte pulse pressure. By precisely adjusting the pressure in the cardiomyocyte culture chamber and observing the corresponding beating conditions of the cardiomyocytes, a device for measuring the pulse pressure of the tissue-engineered cardiomyocytes was designed and developed, especially the systolic pressure, which is an important mechanical performance index of the cardiomyocytes. and measurement of diastolic blood pressure. It provides a new method for measuring the mechanical properties of tissue engineered cardiomyocytes in the field of biomedical engineering. Background technique [0002] Cell therapy has broad prospects for the treatment of myocardial infarction and end-stage heart disease, but the current clinical effect is not very satisfactory. One of the main reasons is that the mechanical properties of cardiomyocyt...
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IPC IPC(8): C12M1/34G01N7/00
CPCG01N7/00
Inventor 李宏求冰霞
Owner HANGZHOU BAIQIAO MEDICAL TECH CO LTD