In-vitro chrysanthemum breeding method

Abstract

The invention discloses an in-vitro chrysanthemum breeding method, belongs to the technical field of biotechnology, and in particular relates to an in-vitro breeding method for chrysanthemum, for mainly solving the problem that the chrysanthemum in the blossoming period is likely to be slimsy when being affected by the environment. The breeding method comprises the following steps: 1, adding a NAA (Naphthalene Acetic Acid) solution and a 6-BA solution into an MS (Murashige / Skoog) solution to prepare a breeding culture liquid; 2, treating stems of chrysanthemum in the blossoming period, washing in clean water and subsequently sterilizing to obtain treated chrysanthemum stems; 3, putting the chrysanthemum stems into an incubator with the breeding culture liquid, controlling the lighting intensity and the lighting time, and touching pollen for artificial pollination; and 4, collecting mature seeds, and naturally drying at a cool place so as to accomplish the in-vitro breeding of chrysanthemum. According to the invention, the breeding process is carried out in the incubator, so that external geographical conditions are avoided, and the ripening rate for in-vitro chrysanthemum culturing is 5%. The method is mainly used for chrysanthemum breeding.

Description

technical field [0001] The invention belongs to the field of biotechnology breeding, and in particular relates to an in vitro breeding method for chrysanthemums. Background technique [0002] Chrysanthemum (Dendranthema grandiflourm Kitam) is native to my country, and because of its rich colors and varieties, it is very popular among the public and widely planted all over the world. There are more than 7,000 chrysanthemum varieties in the world today, and there are more than 3,000 chrysanthemum varieties in my country. Due to the development of the flower market and the improvement of people's appreciation level, the demand for chrysanthemum varieties is also diversified, requiring people to continuously cultivate new varieties. The new breeding technology is also playing an increasingly important role in the selection of new varieties. Traditional breeding methods have been unable to meet the needs of the market. Breeding methods such as mutation breeding, molecular breeding...

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a chrysanthemum isolated breeding method in order to solve the problem that the existing chrysanthemums in the flowering stage are easily affected by the environment and cause no fruit.