Emodin freeze-dried powder and preparation method thereof
A technology for freeze-dried powder injection and emodin, applied in the field of freeze-dried powder injection of emodin and its preparation, can solve the problems of slow absorption, delayed treatment timing, patient loss and the like
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Embodiment 1
[0014] Dosing: Weigh 500.00g of emodin, put it in a container, add 100g of mannitol, 10g of sodium bisulfite, 0.5g of EDTA-2Na, add water to dissolve, adjust the pH to 10.5 with sodium hydroxide solution, add water to 10000ml , stir evenly;
[0015] Filtration and packaging: filter with 0.22μ microporous membrane, and pack the filtrate into 2.0 ml per bottle; freeze-drying: cool down at a rate of 5°C per minute until the temperature of the product drops to -40°C, and maintain for 3 hours; start vacuuming , control the vacuum degree at 100±50μbas, control the product temperature at -20℃, and maintain it for 15 hours; then, gradually increase the temperature to 20℃, control the vacuum degree at 200±50μbas, after reaching 20℃, continue to maintain it for 3 hours, and vacuum plug , that is.
Embodiment 2
[0017] Dosing: Weigh 100.00g of 2-hydroxypropyl-β-cyclodextrin, add water to dissolve it, weigh 100.00g of emodin, put it in the above solution, and stir evenly; then add 400g of lactose and 3.0g of sodium thiosulfate , EDTA-2Na0.5g, stir to dissolve, adjust pH to 9 with sodium hydroxide solution, add water to 10000ml, stir evenly; filter and pack: use 0.22μ microporous filter membrane to filter, filtrate is divided into 1.0 ml per bottle ;Freeze-drying: Cool down at a rate of 5°C per minute until the product cools to -40°C and maintain for 3 hours; start vacuuming, control the vacuum at 100±50μbas, control the product temperature at -20°C, and maintain for 15 hours; then , gradually raise the temperature to 20°C, control the degree of vacuum at 200±50μbas, after reaching 20°C, continue to maintain it for 3 hours, and vacuum stopper.
Embodiment 3
[0019] Dosing: Weigh 50.00g of 2-hydroxypropyl-β-cyclodextrin, add water to dissolve, weigh 100.00g of emodin, put it in the above solution, and stir well; then add mannitol 400g, sodium thiosulfate 5.0 g, EDTA-2Na0.5g, stir to dissolve, adjust the pH to 9.5 with sodium hydroxide solution, add water to 10000ml, and stir evenly; filter and pack: use 0.22μ microporous filter membrane to filter, and divide the filtrate into 2.0 ml each Packing; freeze-drying: cool down at a rate of 5°C per minute until the product cools to -40°C, and maintain for 3 hours; start vacuuming, control the vacuum degree at 100±50μbas, and control the product temperature at -20°C, and maintain for 15 hours; Then, gradually raise the temperature to 20°C, control the degree of vacuum at 200±50μbas, after reaching 20°C, continue to maintain it for 3 hours, and vacuum stopper to obtain the product.
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