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Breeding method of selenium-enriched marine microalgae and its application in feed immune enhancer

A technology of marine microalgae and breeding methods, applied in the direction of microorganism-based methods, applications, animal feed, etc., can solve the problems of inorganic selenium toxicity, dosage and range limitations, etc., achieve easy source of raw materials, save space, and reduce usage Effect

Inactive Publication Date: 2016-05-25
江苏好润生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Selenium has so many biologically active functions such as anti-aging, anti-oxidation, and enhancing immunity, but inorganic selenium is toxic, and its dosage and range are strictly limited

Method used

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  • Breeding method of selenium-enriched marine microalgae and its application in feed immune enhancer

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] 1. Preparation of algae species: After culturing seawater chlorella to the logarithmic phase, move it from a 250mL conical flask to a 3L conical flask for expanded cultivation. The inoculation density is 40mL / L, the ventilation rate is 2.0L / min, and the temperature is controlled at 23°C ±2°C, light intensity 3000lx, light-dark ratio 12h:12h, culture medium f / 2, shake the culture bottle regularly 3 times a day, and culture for 2 days.

[0030] 2. Configure the solid medium: mix 65wt% bran, 33wt% soybean meal, 1wt% lime and KH 2 PO 4 1wt% mixed, add 0.02wt% of selenium-enriched powder, every 100 kilograms of the formula of the above ratio, add 83 kilograms of underground seawater with a salinity of 35wt‰ filtered by double-layer 200 mesh cotton cloth and stir evenly, put it into a steamer and put it into a sterilized Cook in the pot for 120 minutes, maintain the temperature at 125°C and the air pressure at 0.01-0.02MPa, cool the cooked medium to 23°C, and set aside;

[...

Embodiment 2

[0040] 1. Preparation of algae species: After culturing seawater chlorella to the logarithmic phase, move it from a 250mL conical flask to a 3L conical flask for expanded cultivation. The inoculation density is 40mL / L, the ventilation rate is 2.0L / min, and the temperature is controlled at 23°C ±2°C, light intensity 3000lx, light-dark ratio 12h:12h, culture medium f / 2, shake the culture bottle regularly 3 times a day, and culture for 2 days.

[0041] 2. Configure the solid medium: mix 88wt% bran, 8wt% soybean meal, 2wt% lime and KH 2 PO 4 2wt% mixed, add 0.05wt% of selenium-enriched powder, every 100 kg of the formula of the above ratio, add 75 kg of underground seawater with a salinity of 34wt‰ filtered by double-layer 200-mesh cotton cloth and stir evenly, put it into a steamer and put it into a sterilized Cook in the pot for 120 minutes, maintain the temperature at 125°C and the air pressure at 0.01-0.02MPa, cool the cooked medium to 23°C, and set aside;

[0042] 3. Inocul...

Embodiment 3

[0051] 1. Preparation of algae species: After culturing seawater chlorella to the logarithmic phase, move it from a 250mL conical flask to a 3L conical flask for expanded cultivation. The inoculation density is 40mL / L, the ventilation rate is 2.0L / min, and the temperature is controlled at 23°C ±2°C, light intensity 3000lx, light-dark ratio 12h:12h, culture medium f / 2, shake the culture bottle regularly 3 times a day, and culture for 2 days.

[0052] 2. Configure the solid medium: 82wt% bran, 15wt% soybean meal, 1.5wt% lime KH 2 PO 4 1.5wt% mixed, adding 0.1wt% of selenium-enriched powder, every 100 kilograms of the formula of the above ratio, adding 50 kilograms of underground seawater with a salinity of 30wt‰ filtered by double-layer 200 mesh cotton cloth and stirring evenly, put into a steamer and put Cook in the bacteria pot for 120 minutes, maintain the temperature at 125°C and the air pressure at 0.01-0.02MPa, cool the cooked medium to 23°C, and set aside;

[0053] 3. I...

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Abstract

The invention discloses a novel immunopotentiator which produces microalgae rich in an immunity active ingredient selenium on a large scale by utilizing hybrid-breeding manner under a solid-state condition, and is applied in livestock feed. Selenium-rich marine microalgae is cultivated by adopting a solid-state culture medium mainly with subsidiary agricultural products bran and bean pulp, and the selenium-rich microalgae powder is crushed into fineness of 200 meshes; selenium-rich microalgae powder is added in the livestock feed according to the amount of 0.1wt%-0.6wt% to feed the livestock. According to the invention, microalgae is cultivated on a large scale by adopting a three-dimensional way and solid culture medium, so that space can be saved, and collecting efficiency of the microalgae is improved; by adding the selenium-rich microalgae powder, livestock weight gain is improved by 6%-8%, feed conversion rate is improved by 10% and usage amount of antibiotics is reduced by 20%.

Description

technical field [0001] The invention belongs to the interdisciplinary field of marine microalgae and livestock and poultry breeding industry, and relates to a large-scale production of microalgae rich in immune active ingredient selenium by using a concurrent culture method under solid state conditions and applying it to livestock and poultry feed to form a new Immune booster. Background technique [0002] Livestock and poultry immune enhancers, also known as immune adjuvants, are substances that increase the body's specific response to antigens or microorganisms through non-specific ways, and can improve the immune response of livestock and poultry bodies. Since Gaston Ramon, a French immunologist and veterinarian, discovered in 1925 that adding certain irrelevant substances to the vaccine could specifically enhance the body's resistance to diphtheria and tetanus toxins, the development of immune enhancers has become one of the most important aspects of biological product r...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/12A23K10/18C12R1/89
Inventor 成明王洪斌朱新迎
Owner 江苏好润生物科技有限公司