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Ethyl acetate extract product of gentrin knotweed, preparation method and application thereof

A technology of ethyl acetate extraction and ethyl acetate extraction, which is applied in the field of ethyl acetate extract of Polygonum cuspidatum and its preparation, can solve the problems that there is no ethyl acetate extract, and achieve the prevention and treatment of senile dementia and the inhibition of butyrylcholine Effect of esterase activity

Active Publication Date: 2014-06-25
INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But, so far, there is no report about the ethyl acetate extract of Polygonum cuspidatum and its application in the preparation of inhibitory butyrylcholinesterase

Method used

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  • Ethyl acetate extract product of gentrin knotweed, preparation method and application thereof
  • Ethyl acetate extract product of gentrin knotweed, preparation method and application thereof
  • Ethyl acetate extract product of gentrin knotweed, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Example 1: Ethyl acetate extract of Polygonum cuspidatum

[0046] 1. Pulverize the knotweed into coarse powder, weigh 500g of knotweed, soak it in 1500ml of petroleum ether three times the weight of the knotweed for 24 hours, and percolate with petroleum ether until the color of the percolate becomes lighter (2500ml of petroleum ether leachate is obtained), recycling residue;

[0047] 2. Drain the solvent in the residue, then extract the residue with 2500ml of 80% ethanol for 2 times, each extraction time is 2h, combine the two alcohol extracts, concentrate it under reduced pressure until there is no alcohol smell, and then mix with etc. Volume of ethyl acetate was extracted, the supernatant was collected, and the extract was evaporated to dryness to obtain 6.69 g of ethanol extract and ethyl acetate extract (yield 1.34%).

Embodiment 2

[0048] Example 2: Ethyl acetate extract of Polygonum cuspidatum

[0049] 1. Pulverize the knotweed into coarse powder, weigh 500g of knotweed, soak it in petroleum ether 5 times the volume (2500ml) of the weight of knotweed for 36 hours, and permeate with petroleum ether until the color of the percolating liquid becomes lighter (obtain 3500ml of filtrate), the residue is recovered;

[0050] 2. Drain the solvent in the residue, then extract the residue twice with 3500ml of 90% ethanol, the extraction time is 3 hours and 2 hours in turn, combine the extracts, and then concentrate until there is no alcohol smell, and then mix with an equal volume of ethanol. Extract with ethyl acetate, collect the supernatant, evaporate to dryness to obtain 4.014 g of ethyl acetate extract (yield: 0.803%).

Embodiment 3

[0051] Example 3: Ethyl acetate extract of Polygonum cuspidatum

[0052] 1. Crushed Polygonum cuspidatum into coarse powder, take 500g of it and soak it in petroleum ether with 3 times the volume (2000ml) of the weight of Polygonum cuspidatum for 18 hours, and percolate until the color of the leachate becomes lighter (3000ml of petroleum ether leachate is obtained), and the residue is recovered. ;

[0053] 2. Drain the solvent in the residue, then extract the residue with 1500ml of 70% ethanol for 3 times, the extraction time is 2 hours, 1.5 hours, 1.5 hours in turn, combine the extracts, and concentrate them under reduced pressure until there is no alcohol smell , and then extracted with an equal volume of ethyl acetate, the supernatant was collected and evaporated to dryness to obtain 5.352 g of ethyl acetate extract (1.07% yield).

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PUM

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Abstract

The invention relates to an ethyl acetate extract product of gentrin knotweed, a preparation method and an application thereof, the ethyl acetate extract product of the gentrin knotweed is prepared by the following steps: immersing gentrin knotweed by petroleum ether and percolating, recovering residues, volatilizing a solvent in the residues to dry, then extracting by ethanol, concentrating the extract to no alcohol taste, then extracting by using ethyl acetate with same volume of a concentrate, collecting an upper layer extract, evaporating to obtain the ethyl acetate extract product. The ethyl acetate extract product of the gentrin knotweed can prevent and treat senile dementia.

Description

technical field [0001] The invention relates to an extract of Polygonum cuspidatum, in particular to an ethyl acetate extract of Polygonum cuspidatum and a preparation method and application thereof. Background technique [0002] Alzheimer's disease is a neurodegenerative disease that seriously threatens human health. Alzheimer's disease (AD) is a major Alzheimer's disease, manifested as memory, comprehension, calculation, judgment, orientation, language, abstract thinking, self-control and other impairments, resulting in the loss of patients' ability to live and work independently. With the acceleration of social aging, AD has become the fourth leading cause of human death. my country is about to enter an aging society, and the disease is gradually showing a younger trend, and the incidence rate is increasing year by year. So far, there is no effective way to cure Alzheimer's disease, but taking acetylcholinesterase inhibitor (ACHEI) is the most effective way to treat Alzh...

Claims

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Application Information

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IPC IPC(8): A61K36/704A61P25/28G01N30/02G01N30/36
Inventor 陈士林黄林芳王冬梅刘艾林卞兆祥徐宏喜杨大坚陈新滋
Owner INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI
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