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Whole genome sequence of babylonia lutosa mitochondria and amplification primers thereof

A whole genome, Ni Dongfeng snail technology, applied in the field of aquatic animal genetics and breeding

Inactive Publication Date: 2014-07-02
HUNAN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there is no report on the mitochondrial genome sequence, genetic and germplasm resources identification and protection of Nidongfeng snail

Method used

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  • Whole genome sequence of babylonia lutosa mitochondria and amplification primers thereof
  • Whole genome sequence of babylonia lutosa mitochondria and amplification primers thereof
  • Whole genome sequence of babylonia lutosa mitochondria and amplification primers thereof

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Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0051] Using the extracted Nidongfeng snail genome DNA as a template, 14 pairs of primers in this application were used for PCR amplification. The PCR products were detected by 1.0% agarose electrophoresis and then sent to the biological company for sequencing. The sequencing results were spliced ​​using biological software ,Specific steps are as follows:

[0052] 1.10×TBE Buffer preparation

[0053] Weigh tromethamine 108g, Na2EDTA.2H2O7.44g, and boric acid 55g into a 1L beaker; add about 800ml of deionized water and stir evenly; adjust the pH to 8.3 with NaOH, add deionized water to 1L, and store at room temperature , diluted 10 times when used, that is, 10× TBE buffer solution.

[0054] 2. DNA extraction

[0055] Genomic DNA Extraction Kit from Marine Animal Tissue (Tiangen Biochemical: DP324) was used to extract the DNA of snail snail, the steps are as follows:

[0056] (1) Take 30 mg of Dongfeng snail gastropod muscle tissue and put it into a centrifuge tube filled wit...

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Abstract

The invention discloses a whole genome sequence of babylonia lutosa mitochondria and amplification primers thereof, and relates to a mitochondria whole genome sequence and amplification primers thereof. The invention provides a material basis for conservation genetics, evolution genetics and marker-assisted selection of excellent breeding varieties of babylonia lutosa by determining the whole genome sequence of babylonia lutosa mitochondria and the amplification primers thereof. The whole genome sequence and the genetic structure of babylonia lutosa mitochondria are shown as a figure 1 and a table 1. The amplification primers of the whole genome sequence of babylonia lutosa mitochondria are 14 pairs.

Description

technical field [0001] The technology belongs to the technical field of aquatic animal genetics and breeding, and is applied to the genetic variation analysis of Nidongfeng spiral mitochondria, identification and protection of germplasm resources, and phylogenetic research. Background technique [0002] Babylonia lutosa (Babylonia lutosa), commonly known as yellow snail, belongs to mollusk Gastropoda, Neog astopoda, Buccinidae, Babylonia (Wang Rucai, 1988), in China Mainly distributed in Fujian, Guangdong, Guangxi, Hainan and other places, it is euryhaline and euthermic shellfish. Nidongfeng snail meat is delicious, nutritious, crisp and refreshing. In recent years, it has become a very popular high-quality marine shellfish in domestic and foreign markets. With its advantages of fast growth and high price, it has now been developed into a new species of mariculture. Due to seawater pollution and overfishing by human activities, the habitat and germplasm sources of Nidongfen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/11
Inventor 王晓清熊钢曾志南秦溱巫旗生朱丹丽
Owner HUNAN AGRICULTURAL UNIV
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