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Method for effective preparation of salvianolic acid B extract

A technology of salvianolic acid and extract, which is applied in the field of extraction and separation and purification of natural products, can solve the problems of large amount of extracted impurities, excessive degradation of salvianolic acid B, and low purity of the extract, and achieve high physiological activity and salvianolic acid The effect of high acid B content

Active Publication Date: 2014-07-16
TIANJIN TASLY MORDEN TCM RESOURCES
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The deficiencies of the prior art are that extraction with water has a large amount of impurities extracted, more salvianolic acid B is degraded, industrialization is difficult, and the purity of the salvianolic acid B extract is low; especially for the extraction of salvia miltiorrhiza after high alcohol extraction There are few studies on the method of salvianolic acid B component

Method used

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  • Method for effective preparation of salvianolic acid B extract
  • Method for effective preparation of salvianolic acid B extract

Examples

Experimental program
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Effect test

Embodiment 1

[0050] (1) Take the Danshen medicinal residues extracted with 80% ethanol solution, add pH 5, and extract twice with 50% ethanol solution (the amount of solvent is 5 times the amount of dry medicinal residues / time, and the extraction time is 1.5 hours / time), extract liquid filtration, combined, and discarded medicinal residues;

[0051] (2) The specific gravity of the extract below 60°C is 0.95 (56°C) to obtain a concentrate; the concentrate is cooled to below 20°C to obtain a cooled concentrate;

[0052] (3) Stir the cooled concentrated solution and add 10% hydrochloric acid solution to adjust the pH value to 1.96, carry out acid precipitation, refrigerate below 10°C for 13 hours, and filter the pulp plate frame to obtain the filtrate;

[0053] (4) The filtrate is separated and purified by column chromatography: ①The filler is AB-8 resin, and the dosage is the ratio of resin swelling volume (ml) to medicinal material weight (g) 1:1; ②The flow rate of the sample is 1BV-3BV res...

Embodiment 2

[0056] (1) Take the dried rhizome of Salvia miltiorrhiza and extract the wet medicine dregs with 85% ethanol, and add the ethanol solution with a pH value of 2 and an alcohol content of 30% to extract once (the amount of solvent is 2 times (v / w) dry medicine amount of dregs, the extraction time is 0.5 hours), the extract is filtered, and the dregs are discarded;

[0057] (2) Concentrate the extract at 40°C to a specific gravity of 0.8 (56°C) to obtain a concentrated solution; cool down to 0°C to obtain a cooled concentrate;

[0058] (3) Stir the cooled concentrated solution and add 10% (v / v) sulfuric acid solution to adjust the pH value to 1, carry out acid precipitation, leave the concentrated solution at 2°C for 1 hour, and filter the pulp plate frame to obtain the filtrate;

[0059] (4) The filtrate is separated and purified by column chromatography: ①The filler is D101 resin, and the dosage is the ratio of resin swelling volume to the amount of medicinal material 0.5:1 (v / ...

Embodiment 3

[0062] (1) Take the wet dregs of fresh salvia miltiorrhiza after being extracted with 90% ethanol solution, add PH value to 8, and extract 5 times with ethanol solution with 60% alcohol content (the amount of solvent is 15 times (v / w) dry The amount of dregs / time, the extraction time is 5 hours / time), the extract is filtered and combined, and the dregs are discarded;

[0063] (2) Concentrate the extract at 100°C to a specific gravity of 1.20 (56°C) to obtain a concentrated solution; cool down to 25°C and place it for 24 hours to obtain a cooled concentrate;

[0064] (3) Stir the cooled concentrated solution and add 10% (v / v) acetic acid solution to adjust the pH value to 3, carry out acid precipitation, leave the concentrated solution at 20°C for 48 hours, and filter the pulp plate frame to obtain the filtrate;

[0065] (4) The filtrate is separated and purified by column chromatography: ①The filler is polyamide resin, and the dosage is the ratio of resin swelling volume to th...

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Abstract

The invention discloses a method for effective preparation of a salvianolic acid B extract. The method comprises the steps of: (1) taking Salvia Miltiorrhiza dregs extracted by a 80-100% ethanol solution, adding an ethanol solution with a pH value of 2-8 and an alcohol content of 30-60% to conduct extraction so as to obtain an extracted solution; (2) concentrating the extracted solution to obtain a concentrated solution, and conducting cooling; (3) subjecting the concentrated solution to acid precipitation, carrying out low-temperature standing and solid-liquid separation, thus obtaining a liquid; (4) separating and purifying salvianolic acid B by column chromatography; and (5) carrying out concentration and drying to obtain the salvianolic acid B extract.

Description

technical field [0001] The invention belongs to the technical field of extraction, separation and purification of natural products, and relates to a method for extracting and purifying salvianolic acid B from salvia miltiorrhiza, in particular to extracting and purifying salvianolic acid B from medicinal dregs of salvia miltiorrhiza after high-alcohol extraction Methods. Background technique [0002] Salvia miltiorrhiza is the dry root and rhizome of Salvia miltiorrhiza Bge. It is a perennial herb and is one of the commonly used medicines in traditional Chinese medicine. Clinically, it is mainly used to treat kidney disease, liver disease, cardiovascular and cerebrovascular diseases, etc., and has a wide range of application fields. [0003] The dregs of salvia miltiorrhiza after high-alcohol extraction still contain a large amount of salvianolic acid B component. Pharmacological studies have shown that salvianolic acid B has strong pharmacological effects in scavenging fr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D307/86
CPCC07D307/86
Inventor 刘岩范开黄松贾佳丽
Owner TIANJIN TASLY MORDEN TCM RESOURCES
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