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A kind of illumination-magnesium ion double cross-linked alginate hydrogel and preparation method thereof

An alginate and hydrogel technology, which is applied in the field of biomedicine, can solve the problems of poor biological activity and poor stability of photo-crosslinked alginate hydrogels, and achieves the benefits of proliferation, enhanced adhesion, and controllability. sexual effect

Active Publication Date: 2016-03-02
WUHAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In order to overcome the shortcomings of poor stability of ion-crosslinked alginate hydrogel and poor biological activity of photo-crosslinked alginate hydrogel, the present invention provides a stable, controllable, and biologically active light-magnesium ion double-crosslinked Linked alginate hydrogel and preparation method thereof

Method used

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  • A kind of illumination-magnesium ion double cross-linked alginate hydrogel and preparation method thereof
  • A kind of illumination-magnesium ion double cross-linked alginate hydrogel and preparation method thereof
  • A kind of illumination-magnesium ion double cross-linked alginate hydrogel and preparation method thereof

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Experimental program
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Effect test

Embodiment 1

[0031](1) First prepare photocrosslinkable alginate according to the method reported in the literature (Jeon, O., etal.Biomaterials, 2009.30(14):p.2724-34): Dissolve 4g of alginate in 400mL of chloride Sodium / 2-(N-morpholine)ethanesulfonic acid solution (concentration of sodium chloride is 0.5M, concentration of 2-(N-morpholine)ethanesulfonic acid is 50mM), magnetically stirred overnight to prepare 1% (w / v) alginate solution; at room temperature, titrate the alginate solution to pH=6.5 with 5M sodium hydroxide solution, then add N-hydroxyl-sulfosuccinimide and 1-ethyl-(di Methylaminopropyl)-carbon-containing nitroglycerin to activate the carboxyl group on the alginate, after standing for 5min, add 15.8g: 2-aminoethyl methacrylate hydrochloride; wherein, N-hydroxyl-thio Succinimide: 1-ethyl-(dimethylaminopropyl)-carbon-containing nitroglycerin: 2-aminoethyl methacrylate hydrochloride is added in a molar ratio of 1:2:1; at room temperature After reacting for 1 day, pour the re...

Embodiment 2

[0036] (1) Prepare the gel precursor solution of Mg-1: dissolve the photocrosslinkable alginate in the proportion of 2.5% (w / v) in deionized water containing 0.0625% (w / v) photoinitiator I2959 ; The preparation concentration is the MgSO of 5mmol / L 4 solution, MgSO 4 The solution is uniformly mixed with the above alginate solution at a volume ratio of 1:4 to obtain a gel precursor solution. The final concentration of each component is as follows: photocrosslinkable alginate, 2% (w / v); I2959 , 0.05% (w / v); MgSO 4 , 1mmol / L.

[0037] (2) Drop the gel precursor solution into the mold, let it stand for 30 minutes, and then place it at a wavelength of 365nm and an intensity of 8mW / cm 2 Irradiate under the ultraviolet lamp for 10min to obtain light-magnesium ion double cross-linked alginate hydrogel (Mg-1).

Embodiment 3

[0039] (1) Prepare the gel precursor solution of Mg-10: dissolve the photocrosslinkable alginate in the proportion of 2.5% (w / v) in deionized water containing 0.0625% (w / v) photoinitiator I2959 ; The preparation concentration is the MgSO of 50mmol / L 4 solution, MgSO 4 The solution is uniformly mixed with the above alginate solution at a volume ratio of 1:4 to obtain a gel precursor solution. The final concentration of each component is as follows: photocrosslinkable alginate, 2% (w / v); I2959 , 0.05% (w / v); MgSO 4 , 10mmol / L.

[0040] (2) Drop the gel precursor solution into the mold, let it stand for 30 minutes, and then place it at a wavelength of 365nm and an intensity of 8mW / cm 2 Irradiate under the ultraviolet lamp for 10min to obtain light-magnesium ion double cross-linked alginate hydrogel (Mg-10).

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Abstract

The invention discloses an illumination-magnesium ion double-crosslinking alginate hydrogel and a preparation method thereof. The illumination-magnesium ion double-crosslinking alginate hydrogel can be prepared by preparing a precursor solution via an alginate solution with photo-crosslinking function, a photoinitiator and magnesium ions with bioactivity and carrying out of ionic reaction and ultraviolet illumination double-crosslinking reaction. According to the illumination-magnesium ion double-crosslinking alginate hydrogel and the preparation method thereof, the magnesium ions are imported into a bracket through an ion crosslinking manner, so that the biological characteristic of the bracket is simply and effectively improved; at the same time, a main framework is formed through photo-crosslinking, so that the stability of the bracket is ensured; and through ultraviolet initiation and the slow crosslinking characteristic of the magnesium ions, the favorable controllability of the bracket is ensured. The preparation method is simple and does not need to import other materials; and through controlling the concentration of the magnesium ions, the physical and chemical properties and the biological property of the hydrogel can be effectively adjusted and convenience is brought to the practical application.

Description

technical field [0001] The invention belongs to the field of biomedicine, and relates to an alginate hydrogel and a preparation method thereof, in particular to an illumination-magnesium ion double-crosslinked alginate hydrogel and a preparation method thereof. Background technique [0002] Bone defects caused by trauma, tumors, infections, and abnormal development have always been a difficult point in clinical treatment. Bone transplantation is the main method for reconstructing defective bone tissue, but when using autologous bone transplantation, the source of autologous bone is less, and it will bring some pain to the patient; when using allogeneic bone transplantation, there are problems such as infection and rejection. The development of tissue engineering provides a new way of thinking for the development of ideal bone grafts. Scaffold is one of the three elements of bone tissue engineering. In addition to non-toxicity, non-immunogenicity, degradability and good mech...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/04C08J3/28C08J3/24
Inventor 王家伟尹苗徐飞邓婷
Owner WUHAN UNIV