Marker for indicating hepatitis infection and application of marker

A technology for markers, hepatitis, applied in the fields of biotechnology and medicine

Inactive Publication Date: 2014-07-16
ANHUI PROVINCIAL HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there is no report on the use of sST2 as a serum marker of hepatitis virus infection at home and abroad

Method used

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  • Marker for indicating hepatitis infection and application of marker
  • Marker for indicating hepatitis infection and application of marker

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0041] The blood of hepatitis patients before and after treatment and the blood of normal healthy volunteers were collected. The experiment was carried out in Anhui Provincial Hospital. All sera tested were collected from hepatitis patients before and after treatment and blood from normal healthy volunteers in the Department of Infectious Diseases and Physical Examination Center. Centrifuge at 3000 rpm at room temperature, take the serum and store it in an EP tube at -80°C. 100 μl of serum was taken to detect the content of sST2 in serum by ELISA method. The Duoset ELISA kit of R&D Company was used for detection, and all reagents were placed at room temperature before use. The detection was carried out in full accordance with the instructions of the kit. Specifically include:

[0042] Capture antibody: reconstitute mouse anti-ST2 antibody with 1.0ml PBS to 360μg / ml, which can be stored at 2°C-8°C for 60 days or at -20°C-70°C for 6 months, when in use To be diluted with PB...

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Abstract

The invention provides a marker for indicating hepatitis infection, application of the marker for preparing drugs for detecting or treating hepatitis infection and application of the marker in a kit, wherein the marker is a mark substance for characterizing an amount of ST2. The invention further relates to soluble ST2 which is a marker for reflecting inflammation of patients with hepatitis and reflecting serum, a method for measuring the content of the serum and application of the soluble ST2 in diagnosis and treatment of hepatitis. Change of the content of the ST2 in the serum is detected by an ELISA (Enzyme-Linked Immunosorbent Assay) method; the soluble ST2 is significantly increased in the serum of the patients with the hepatitis; the more serious the inflammation degree of the patients with the hepatitis, the higher the concentration of the ST2; conversely, if the treatment effect is better, the concentration of the ST2 is gradually reduced; the marker can serve as a serum marker for noninvasively, simply, conveniently and quickly diagnosing the hepatitis and judging development of course of disease.

Description

technical field [0001] The invention belongs to the fields of biotechnology and medicine. It involves a new serum marker of hepatitis virus infection. Specifically, the present invention relates to a serum marker soluble ST2 that can reflect organ inflammation in patients with hepatitis, a method for measuring the serum content of the marker and its use in hepatitis diagnosis and treatment. Background technique [0002] my country is a high incidence area of ​​viral hepatitis. The prevalence rate of hepatitis A population (anti-HAV (hepatitis A virus) positive) is about 80%. There are about 350 million HbsAg (hepatitis B surface antigen) carriers in the world, including about 120 million in my country. There are about 170 million HCV-infected people in the world, and 3.2% of the anti-HCV-positive people in my country, about 30 million. The prevalence of hepatitis D in the population is about 1%, and that of hepatitis E is about 17%. With the development of hepatitis, am...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/576G01N33/68C12Q1/68
CPCG01N33/576C12Q1/6883G01N33/6869
Inventor 沈国栋胡世莲沈干程民王璐黄毕林
Owner ANHUI PROVINCIAL HOSPITAL
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