Identification genes, primers and detection methods of Solanum species

Abstract

The invention provides a gene to identify solanum species, wherein the gene is composed of a main gene sequence namely a nuclear gene waxy and other auxiliary genes namely chloroplast genes such as trnS-trnG, ndhF, and the like. The invention also provides a sequence synthetic primer of the nuclear gene waxy, and further provides a detection method for identifying four solanum weeds and other alien solanum weeds for entry quarantine. The gene has a good specificity, and the detection method has the advantages of quickness, simpleness, high accuracy, and high sensitivity. A method is provided for solanum species detection, and thus the entry of alien solanum species is prohibited.

Description

technical field [0001] The invention relates to the technical field of biological detection, in particular to a molecular identification gene sequence of a species of Solanum genus, a PCR primer and a detection method thereof. Background technique [0002] There are more than 1,400 species of Solanum in the world, most of which originated in South America. They are widely distributed and adaptable. Some invasive weeds are very harmful. Among them, 4 species have been listed as entry quarantine weeds in my country, and another 30 species The rest are potentially harmful. However, the morphological variation of this group is complex, and species identification is extremely difficult, especially for the identification of alien species. [0003] DNA barcoding technology can overcome the problem of accurate identification of species due to factors such as lack of sufficient trait information and phenotypic plasticity, so this technology has become the most promising development d...

AI Technical Summary

Problems solved by technology

However, no gene sequence that can be used as a universal DNA with strong resolution and can be used as a barcode has been found so far in terrestrial plants

The main reason is that the evolution rate of the plant mitochondrial genome is slow and the genetic variation is small, so the mitochondrial COI sequence is not suitable for plants; in addition, the heterogeneity of hybridization, network evolution and the evolution rate of the same gene in different groups during the evolution of plants Making Plant Barcode Research Much More Complicated Than Animals

In recent years, many scholars from various countries have proposed many candidate sequences and combinations of plant DNA barcodes. For example, the chloroplast gene rbcL+matK combination and the nuclear gene ITS have been proposed as the core barcodes of terrestrial plants, but they can only solve higher-order elements (family, genus) identification problem, the resolution at the species level is poor, such as a single rbcL and matK can only distinguish less than 50% of the species, and the resolution of their combination is less than 70%

Therefore, the universality of the identification of the current research results is not ideal, and none of them meet the application conditions.

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