A method for detecting resistance markers in the growth stage of Atractylodes macrocephala

Abstract

The invention discloses a method for detecting resistance markers of Atractylodes macrocephala during its growth period. The method is to collect susceptible plants and disease-resistant plants in the natural state during the high-incidence period of Atractylodes macrocephala soil-borne diseases; at the same time, adopt the method of root injury to inoculate the mycelia of Sphagnum albicans, and collect healthy and diseased plants respectively after the onset of infection, and use HPLC method The levels of major metabolites Atractylodes atractylodes lactones in the sample plants were measured, the differences between groups were compared, the components with significant differences were determined, and the marker metabolites related to the resistance of Atractylodes macrocephala were screened, and Sclerotium rolfsii Sacc.) is the test bacterium, and the toxicity test of this component is carried out to prove the antibacterial effect of Atractylodes lactone II, so as to obtain resistance markers, which are used for the evaluation of Atractylodes atractylodes resistance, the selection of resistant varieties, and the like.

Description

technical field [0001] The invention relates to a method for detecting resistance markers in the growth stage of Atractylodes macrocephala, in particular to a secondary metabolite atractylodes lactone II used as a marker detection method related to the resistance of Atractylodes rhizome soil-borne diseases. Background technique [0002] Atractylodes macrocephala (Atractylodis macrocephala Koidz.) is one of the bulk Chinese herbal medicines in my country. It has been introduced and cultivated in about 20 provinces (municipalities) across the country, and the current planting area is about 13,000 hectares. However, under the conditions of suitable soil conditions, cultivation conditions and climate conditions, the loss rate of Atractylodes Rhizoma Atractylodes Rhizoma Atractylodes Rhizoma Atractylodes Rhizoma Atractylodes Rhizoma Atractylodes Rhizoma is as high as 50% when serious, and even cause crop failure. Soil-borne diseases of Atractylodes macrocephala are limiting facto...

AI Technical Summary

Problems solved by technology

However, Atractylodes macrocephala, like other medicinal plants, lacks a large amount of sequence data information, making it difficult to apply systematic analysis of gene expression, transcriptional profiling analysis methods such as microarrays, and cDNA-ALFP technology to the expression analysis of genes related to various biological functions. Biological functions include heredity, fertility, and disease resistance of Atractylodes macrocephala, which seriously hinders the large-scale gene discovery of Atractylodes rhizome

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