Device for cell migration experiment
A cell migration and experimental technology, applied in the field of devices for cell migration experiments, can solve the problems of uneven scratch area, uneven scratches, uneven scratch edges, etc., and achieve the effect of accurate area calculation
Active Publication Date: 2014-07-30
QIANFOSHAN HOSPITAL OF SHANDONG
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Problems solved by technology
In the current scratch test, a sterile plastic tip is generally used to scratch manually, such as figure 1 As shown, it can be seen that the edge of the scratch is not neat, there are residual cells in the scratch after washing with PBS, and the area of the scratch is different in different wells, which will affect the experimental effect and the judgment of the result. Inaccurate location of marks, non-uniform scratch area, etc.
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[0017] A device for cell migration experiments, comprising a plate body 1 and holes 2 evenly distributed on the plate body 1, the holes 2 are arranged in a square shape. The number of required holes can be determined according to the demand. A grid 4 is arranged outside the bottom of the hole 2 with a grid spacing of 0.25 mm. The centerline of the grid 4 coincides with the central axis of the hole 2 . On the inner side of the bottom of hole 2, there is a separator 3 made of ultra-thin transparent plastic strips. The adhesive material is a medical polymer adhesive, which is non-cytotoxic. After removing the separator 3, there is no residue at the bottom of hole 2 and does not affect the cells. grow. The longitudinal centerline of the divider 3 coincides with the centerline of the grid 4, so that when the divider 3 is peeled off, the distance between the cells on both sides of the divider 3 relative to the centerline of the grid 4 is the same, which is convenient for statistics....
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Abstract
The invention discloses a device for cell migration experiment and relates to an appliance for a laboratory. The device comprises a plate body and holes uniformly distributed on the plate body, wherein the holes are square; a grid is arranged outside the bottoms of the holes; a center line of the grid is superposed with central axes of the holes; dividing strips are pasted inside the bottoms of the holes; the pasting material is a medical high polymer binder; longitudinal centre lines of the dividing strips are superposed with the center line of the grid; the dividing strips are 1 mm wide, and width of the dividing strips is greater than length of the holes; a holding part is arranged at one end of each of the dividing strips. According to the device for cell migration experiment disclosed by the invention, square holes are designed, and the area of scratches is rectangular, so that area calculation is more precise in comparison with that of circular holes; dividing strips with pulling rings are adhered in the holes, so that the dividing strips can be uncovered to obtain scratches which are consist in size and neat in specification while scratch experiment is carried out; moreover, the device is convenient and quick; the grid is arranged on the bottoms of the holes, so that cell migration positions can be conveniently observed and counted to calculate relative scratch area.
Description
technical field [0001] The invention relates to a laboratory appliance, in particular to a device for cell migration experiments. Background technique [0002] Cell migration is ubiquitous in the normal physiological activities of the human body and in the occurrence of diseases, such as cell foraging, wound healing, embryogenesis, nervous system formation, immune response, cancer metastasis and many other events are involved in cell migration. Cell migration is an important direction and focus of current life science research. Scientists are trying to achieve greater results in medical applications such as vascular injury repair, prevention of cancer metastasis, and skin grafting through the study of cell migration. [0003] At present, cell migration is mainly studied by scratch experiment. Epithelial cells, keratinocytes, etc. form single-layer or stratified epithelium under physiological conditions. Under pathological conditions such as wound healing, cells migrate, mai...
Claims
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IPC IPC(8): C12M1/00
CPCB01L3/50855
Inventor 曹莉莉宋艳王亮孔歉歉吴广萍杜娟郑盈盈郑亚冰
Owner QIANFOSHAN HOSPITAL OF SHANDONG