A method for obtaining measured value of liquid chromatography inspection

A liquid chromatography and measurement technology, applied in the field of medical chromatographic analysis, can solve the problems of asymmetric tailing peaks with large errors, and achieve the effect of reducing errors and small errors

Active Publication Date: 2017-07-18
SHENZHEN LIFOTRONIC TECH
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Problems solved by technology

[0004] The technical problem to be solved by the present invention is to provide a liquid chromatographic test method with relatively small errors when the above-mentioned methods of the prior art are used to segment and quantify the asymmetric tailing peaks. The method of obtaining the measured value of

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  • A method for obtaining measured value of liquid chromatography inspection
  • A method for obtaining measured value of liquid chromatography inspection
  • A method for obtaining measured value of liquid chromatography inspection

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Embodiment Construction

[0043] In order to facilitate those of ordinary skill in the art to understand and implement the present invention, the embodiments of the present invention will be further described below in conjunction with the accompanying drawings.

[0044] In the embodiment of the method for obtaining the measured value of the liquid chromatography test of the present invention, the structural schematic diagram of the method is as follows Figure 4 shown. Figure 4 , the method includes the following steps:

[0045] Step S01 Obtaining the first chromatographic elution curve: In this step, the output first chromatographic elution curve is obtained from the chromatographic detection system.

[0046] Step S02 obtains the baseline from the first chromatographic elution curve: after the operating conditions are stable, when no sample passes through, the signal-time curve reflected by the detector is the baseline, that is, the baseline is the chromatographic elution curve when no sample compon...

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Abstract

The invention relates to a method for obtaining measurement values ​​of liquid chromatography inspection, comprising the following steps: obtaining a first chromatographic outflow curve; obtaining a baseline from the first chromatographic outflow curve; The peak and valley values ​​of the corresponding chromatographic peak; obtain and retain the first curve between the maximum slope point of the ascending section and the minimum slope point of the descending section of the chromatographic peak corresponding to the required component, and the first curve of the chromatographic peak The first rising curve is translated to obtain the second rising curve; in the same way, the first falling curve and the second falling curve are obtained, and then the first chromatographic peak is obtained; the content value of the required component is calculated according to the first chromatographic peak, and is obtained by the same method The content values ​​of other components in the first chromatographic elution curve are obtained, and then the measured values ​​are obtained from the content values ​​of the required components and the content values ​​of other components. The implementation of the measurement value acquisition method for liquid chromatography inspection of the present invention has the following beneficial effects: the introduced error is small.

Description

technical field [0001] The invention relates to the field of medical chromatographic analysis, and more specifically relates to a method for obtaining measurement values ​​of liquid chromatography inspection. Background technique [0002] Glycosylated hemoglobin is the product of the combination of hemoglobin and blood sugar in red blood cells. Its concentration is directly proportional to the blood sugar concentration and can remain in human blood for about 120 days. It can reflect the blood sugar control of the patient in the past 8 to 12 weeks and is an important basis for diagnosing diabetes. one. [0003] At present, the methods for determining glycosylated hemoglobin mainly include ion chromatography, electrophoresis, immunoassay, enzymatic method, etc., and ion chromatography, as one of the most important detection methods, is being used more and more widely, but for ion layer However, the chromatographic elution curve detected by the analytical method has never had ...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/86
Inventor 徐岩刘先成彭国庆胡文雍舒伟
Owner SHENZHEN LIFOTRONIC TECH
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