A ledebouriella root/smoked plum composition for treating asthma and applications of the composition
A composition and technology of ebony, applied in the fields of biology and pharmacy, can solve problems such as compatibility of unseen prescriptions
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[0013] (1) Prepare Fangfeng-black plum extract (prescription ratio is 1:1), and use distilled water to prepare it. The dosage for clinical adults is 30g of crude drug per day, and the equivalent dose is given to mice according to the ratio of the surface area of experimental animals to human body. Select 60 female BALB / C mice (SPF grade), six weeks old, body weight: 18-22 g. Grouping: After numbering according to the body weight of the animals, the animals were divided into 5 groups with a random number table, 12 in each group. The model was made according to the research of Temelkovski et al. On the 0th, 7th, and 14th day of the experiment, 0.2ml of antigen solution (containing 100μg of oval protein OVA and 400μg of aluminum hydroxide) was injected intraperitoneally, and the mouse was placed in a plexiglass container (30×15×20cm) on the 21st day. In this method, 5% OVA solution was nebulized (with a particle diameter of 3-5 μm) for 30 min with an ultrasonic nebulizer to in...
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