Traditional Chinese medicine probiotics compound preparation for preventing and treating respiratory diseases of broilers

A technology of composite preparations and probiotics, which is applied in the direction of respiratory diseases, drug combinations, and medical preparations containing active ingredients, etc., can solve the problems of limited types, high prices, and slow curative effect of traditional Chinese medicine, and achieve slow onset, Effects of avoiding infection, promoting absorption and utilization

CN104688895AActive Publication Date: 2015-06-10山东宝来利来生物工程股份有限公司 +1
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
Publication Date
2015-06-10

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Abstract

The invention discloses a traditional Chinese medicine probiotics compound preparation for preventing and treating respiratory diseases of broilers. The preparation is prepared by compounding a traditional Chinese medicine extraction liquid and bacterial powder of probiotics, wherein the mass concentration of the traditional Chinese medicine extraction liquid is 0.1-1g / mL and the traditional Chinese medicine extraction liquid is prepared from the following medicinal raw materials decocted by water in parts by weight: 14-18 parts of smoked plums, 10-14 parts of pomegranate barks, 6-10 parts of liquorice, 6-10 parts of pericarpium citri reticulatae, 6-10 parts of mangnolia officinalis, 2-6 parts of houttuynia cordata, 2-6 parts of fructus forsythia and 2-6 parts of poria cocos. The probiotics are one of bacillus or lactic acid bacteria or a mixture of the two. The viable count of each kind of probiotics in the traditional Chinese medicine probiotics compound preparation is 10*<5>-10*<9>cfu / mL. The invention further discloses a preparation method of the traditional Chinese medicine probiotics compound preparation. According to the traditional Chinese medicine probiotics compound preparation, traditional Chinese medicines and probiotics are reasonably combined, wherein the traditional Chinese medicines can promote proliferation of probiotics, the probiotics can promote absorption and utilization of traditional Chinese medicines, and the probiotics and the traditional Chinese medicines are combined in use to play a relatively good preventing and treating role.
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Description

technical field

[0001] The invention relates to a traditional Chinese medicine probiotic compound preparation for preventing and treating respiratory diseases of broilers. Background technique

[0002] Poultry respiratory disease has always been a thorny problem in the poultry industry, and it almost occurs in intensive poultry farms in many areas of our country, seriously affecting the economic benefits of the poultry industry. The disease mainly manifests as respiratory symptoms, with a high incidence rate, long course of disease, fast transmission speed, and easy recurrence. Take broiler chickens as an example. Respiratory diseases in broilers will reduce the feed utilization rate of chickens. Although they can be controlled with medication, it is difficult to cure them. Therefore, respiratory diseases are a type of disease that cannot be ignored in poultry production. At present, the treatment of poultry respiratory diseases mainly relies on various western medicines, t...

Examples

Embodiment 1

[0039] Embodiment 1: the isolation of pathogenic bacteria

[0040] 1. Test material:

[0041] 1.1 Disease material: The larynx, trachea, air sac, and lungs of chickens suffering from respiratory diseases around Tai'an City were aseptically collected.

[0042] 1.2 Media: Normal Nutrient Broth Agar Medium (NA), Blood Agar Medium, Eosin-Maylan Agar Medium (EMB), Tryptone Soy Agar Medium (TSA), Tryptone Soy Broth Medium (TSB ), SS medium, MacConkey medium (MAC), and chocolate agar medium, all of which were purchased from Qingdao Haibo Company.

[0043] 0.005% nicotinamide adenine dinucleotide (NAD), purchased from Beijing Land Bridge Technology Co., Ltd.

[0044] 1.3 Biochemical trace identification tubes: lactose, glucose, arginine, xylose-gelatin, MR-VP, malonate, mannitol, dulcitol semi-solid, ONPG, Simon's citrate, urease, lysate Amino acid, sterile liquid paraffin, and Kovacs' indigo matrix kit were all purchased from Qingdao Haibo Company.

[0045] 1.4 Reagents for molec...

Embodiment 2

[0088] Embodiment 2: Drug susceptibility test of traditional Chinese medicine and probiotics

[0089] 1. Test material

[0090] 1.1 Single traditional Chinese medicine: Poria, Pinellia, Anemarrhena, Campanulaceae, Fangfeng, Andrographis paniculata, Black Plum, Forsythia, Pomegranate Peel, Scutellaria, Scutellaria, Qinpi, Daqingye, Huanglian, Magnolia officinalis, Banlangen, Honeysuckle, Chai Hu, licorice, Houttuynia cordata, ephedra. The above-mentioned traditional Chinese medicines were all purchased from Wanning Pharmacy in Taishan District, Tai'an.

[0091] 1.2 Probiotics:

[0092] Bacillus: Bacillus subtilis JYM 841, Bacillus subtilis B7348.

[0093] Lactic acid bacteria: Pediococcus pentosaceae PP, Enterococcus faecalis SRG, Lactobacillus plantarum LP.

[0094] Bacillus subtilis (Bacillus subtilis) JYM841 has been preserved in China Center for Type Culture Collection on January 10, 2014, and the preservation number is CCTCC NO: M 2014012; Bacillus subtilis B7348 (Baci...

Embodiment 3

[0132] Embodiment 3: Screening of Chinese medicine probiotic compound preparation

[0133] 1. Test material

[0134] 1.1 Experimental animals: 80 1-day-old healthy broiler chickens were purchased from Taian Daewoo Breeding Plant.

[0135]1.2 Experimental diet: purchased from Taian Liuhe Feed Factory, the feed type is 510, 511. 1-21 days old were fed 510, 22-42 days old were fed 511.

[0136] 1.3 Test drugs:

[0137] Chinese medicine compound prescription F1, F2 prepared in embodiment 2;

[0138] Probiotics: Lactobacillus plantarum LP bacteria powder, Bacillus subtilis B7348 bacteria powder. The bacterial powder was provided by Shandong Baolai Lilai Biological Engineering Co., Ltd.

[0139] 1.4 Test reagents:

[0140] Serum biochemical index determination reagents: serum factor total protein (TP), albumin (ALB), urea nitrogen (BUN) kits, purchased from Kehua Biological Company.

[0141] 10% (v / v) neutral formalin fixative: anhydrous disodium hydrogen phosphate 6.5g / L, sod...