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Riemerella anatipestifer indirect coagulation antibody detection kit as well as application thereof

A technology of Duck Reemer's and detection kits, which is applied in the direction of biological testing, measuring devices, material inspection products, etc., to achieve the effect of simple and convenient operation

Active Publication Date: 2014-08-27
LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there is no commercial kit for detecting RA serum antibodies, which is blank at home and abroad

Method used

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  • Riemerella anatipestifer indirect coagulation antibody detection kit as well as application thereof
  • Riemerella anatipestifer indirect coagulation antibody detection kit as well as application thereof
  • Riemerella anatipestifer indirect coagulation antibody detection kit as well as application thereof

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Embodiment 1

[0042] The indirect hemagglutination kit for detecting antibody titers of Riemerella anatipestifer (RA) serum types 1, 2 and 10 involved in the present invention includes:

[0043] 1 vial of RA antigen (lyophilized), frozen at -20°C;

[0044] 1 bottle (0.5ml) of standard positive serum, frozen at -20°C;

[0045] 1 vial (0.5ml) of standard negative serum, frozen at -20°C;

[0046] 3 bottles of diluent (10ml / bottle), stored at 4°C.

[0047] The kit can detect 40-50 serums, and the storage period is 1 year.

[0048] The RA strains used to prepare the antigen are field isolates, a total of 3 strains: WF2 (serum type 1, isolated in 2010), WF7 (serum type 2, isolated in 2010), XY6 (serum type 10, isolated in 2010), preserved In the State Key Laboratory of Livestock Disease Pathogen Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences.

[0049] The preparation method of various reagents in the kit of the present invention is:

[0050] 1. In...

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Abstract

The invention discloses a riemerella anatipestifer indirect coagulation antibody detection kit and application thereof. The kit comprises a riemerella anatipestifer indirect coagulation antigen, standard positive serum, standard negative serum and a diluent. The riemerella anatipestifer indirect coagulation antibody detection kit and the application thereof have the beneficial effects that the kit is simple and convenient to operate, can be widely popularized and used in related grassroots veterinarian departments and farms; the kit only needs a few simple consumable materials and incubators when being used detecting samples without any other device, and can be used even in duck farms with relatively simple and rough conditions; the result can be determined intuitively, and can be observed by eyes; professional knowledge is not required for operators, special training is not required, and the detection can be performed only referring to a specification; the kit is low in cost, is economical and practical, and can be accepted by most of users.

Description

[0001] technical field [0002] The invention relates to a detection kit, in particular to a detection kit for Riemerella anatipestifer indirect hemagglutination antibody and application thereof. Background technique [0003] Riemerella anatipestifer ( Riemerella anatipestifer , RA) is the pathogen of Richeriosis anatipestifer, which can cause domestic ducks, geese, turkeys and a variety of poultry and wild birds to become ill. The resulting duck disease is called duck infectious serositis (duck infectious serositis). The more obvious pathological changes in the diseased ducks are fibrinous pericarditis, perihepatitis, air sac inflammation, and salpingitis. Similar pathological changes are also seen in turkeys and other poultry. The disease was first discovered in Long Island, New York, USA in 1932, and then spread rapidly all over the world. The existence of the disease was first confirmed in my country in 1982. At present, it occurs in all duck raising provinces and reg...

Claims

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Application Information

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IPC IPC(8): G01N33/96G01N33/531
CPCG01N33/56911G01N2333/195
Inventor 刘永生郑福英宫晓炜陈启伟
Owner LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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