Fluorescent quantization PCR kit and detection method for detecting cow endometrial cell inflammatory reaction and application of kit

Abstract

The invention provides a fluorescent quantization PCR kit for detecting cow endometrial cell inflammatory reaction. The fluorescent quantization PCR kit comprises a PCR buffer solution, a TaqDNA polymerase mixture, an SYBRGreen dye, and two pairs of primers, wherein the two pairs of primers include a primer 1 and a primer 2, the primer 1 comprises a forward primer 5'-TATGACCAGGGACCAGGTAC-3' and a reverse primer 5'-CTCTGCCCTTAGGGACTCACAG-3', and the primer 2 comprises a forward primer 5'-ATCGGCAATGAGCGGTTCC-3' and a reverse primer 5'-GTGTTGGCGTAGAGGTCCTTG-3'. The kit disclosed by the invention can be simple and fast, and can relatively and quantitatively detect the transcriptional level of an amyloid protein (SAA) gene in a cow endometrial cell, so as to provide reference for quickly judging whether the dairy cow has recessive endometritis.

Description

technical field [0001] The invention belongs to the technical field of PCR amplification, and in particular relates to a specific fluorescent quantitative PCR kit and a detection method for detecting the inflammatory response of cow endometrium cells. Background technique [0002] Polymerase chain reaction (Polymerase Chain Reaction, PCR) is the most commonly used technique in DNA manipulation technology in vitro, which realizes the exponential amplification of target DNA fragments in the reaction solution. Fluorescence quantitative PCR technology combines real-time fluorescence detection technology and computer analysis technology. Specific fluorescent marker substances are added to the PCR reaction system, and the amplification of DNA can be monitored in real time by detecting the change of fluorescence value in the PCR reaction system after each thermal cycle. and obtain the fluorescence quantitative change curve of each sample, and then obtain the number of PCR cycles (C...

AI Technical Summary

Problems solved by technology

[0004] Recessive endometritis in dairy cows is a common obstetrical disease after postpartum recovery. Due to the lack of obvious clinical symptoms, in vitro diagnosis of recessive endometritis in dairy cows is also relatively difficult, and the disease often causes huge losses to the breeding economy.

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