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PCR (polymerase chain reaction) primer pair for identifying fox tissues and/or organs or identifying fox tissues and/or organs in auxiliary manner and application thereof

A primer pair and animal identification technology, which is applied in the determination/inspection of microorganisms, DNA/RNA fragments, recombinant DNA technology, etc., can solve the problem of high cost, inability to detect heat-processed meat, cross-reaction in mixed meat detection, etc. question

Inactive Publication Date: 2014-09-10
CHINA ANIMAL DISEASE CONTROL CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This type of identification technology is greatly affected by the structure of the protein (the part that plays a major role is the antigenic determinant), and the problems that often occur in practical applications include: 1. The thermal processing process will change the structure of the protein (the antigenic determinant). Detect heat-processed meat; 2. Cross-reaction may occur in the detection of mixed meat, because the structure of proteins of different species may have a certain degree of similarity, and the sensitivity of the test depends on the specificity of the prepared antibody
The DNA molecular hybridization method is complicated to operate, and the cost is high, so it is gradually replaced by the PCR method

Method used

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  • PCR (polymerase chain reaction) primer pair for identifying fox tissues and/or organs or identifying fox tissues and/or organs in auxiliary manner and application thereof
  • PCR (polymerase chain reaction) primer pair for identifying fox tissues and/or organs or identifying fox tissues and/or organs in auxiliary manner and application thereof
  • PCR (polymerase chain reaction) primer pair for identifying fox tissues and/or organs or identifying fox tissues and/or organs in auxiliary manner and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Embodiment 1, single-fold PCR detection animal pure muscle sample

[0052] 1. Preparation of PCR primers for identification or auxiliary identification of tissues and / or organs of domestic dogs, foxes, raccoons and minks

[0053]The identification of this step or the PCR primer pair composition of auxiliary identification domestic dog, fox, raccoon dog and mink tissue and / or organ, by the identification of name clf or the PCR primer pair of auxiliary identification domestic dog tissue and / or organ (abbreviation Domestic dog-specific primer pair clf), PCR primer pair named vvf for identification or auxiliary identification of fox tissues and / or organs (referred to as fox-specific primer pair vvf), identification or auxiliary identification of raccoon dog tissues and / or organs named np The PCR primer pair (abbreviation raccoon-specific primer pair np) and the PCR primer pair (abbreviation mink specific primer pair mv) of identifying or assisting the identification of mink...

Embodiment 2

[0073] Embodiment 2, multiplex PCR detection adulterated muscle sample

[0074] 1. Preparation of PCR primers for identification or auxiliary identification of tissues and / or organs of domestic dogs, foxes, raccoons and minks

[0075]The identification of this step or the PCR primer pair composition of auxiliary identification domestic dog, fox, raccoon dog and mink tissue and / or organ, by the identification of name clf or the PCR primer pair of auxiliary identification domestic dog tissue and / or organ (abbreviation Domestic dog-specific primer pair clf), PCR primer pair named vvf for identification or auxiliary identification of fox tissues and / or organs (referred to as fox-specific primer pair vvf), identification or auxiliary identification of raccoon dog tissues and / or organs named np The PCR primer pair (abbreviation raccoon-specific primer pair np) and the PCR primer pair (abbreviation mink specific primer pair mv) of identifying or assisting the identification of mink t...

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PUM

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Abstract

The invention discloses a PCR (polymerase chain reaction) primer pair for identifying fox tissues and / or organs or identifying the fox tissues and / or organs in an auxiliary manner and an application thereof. A PCR primer pair composition for identifying animal tissues and / or organs or identifying the animal tissues and / or organs in an auxiliary manner, which is provided by the invention, is a composition 1, a composition 2 or a composition 3, wherein the composition 1 is formed by clf, vvf and np; the composition 2 is formed by clf and vvf; the composition 3 is formed by vvf and np. The invention also provides the PCR primer pair vvf for identifying the fox tissues and / or organs or identifying the fox tissues and / or organs in the auxiliary manner. The PCR primer pair has the beneficial effects that each single-stranded DNA (deoxyribonucleic acid) in three pairs of primers in the primer pair selects the annealing temperature of 62 DEG C, thus achieving completion of all identification through one-time PCR at the same temperature; the specificity is strong, and the detection process consumes short time.

Description

technical field [0001] The invention relates to a pair of PCR primers for identifying or assisting in identifying fox tissues and / or organs in the field of biotechnology and applications thereof. Background technique [0002] At present, in the domestic production and sales of raw meat and meat products, some unscrupulous traders use adulteration and other means to deceive consumers in order to make huge profits, such as using fox, raccoon and mink meat as dog meat and beef. and lamb etc. Therefore, a set of methods for quickly and accurately identifying meat types and being able to adapt to the identification of mixed meat products will undoubtedly provide strong technical support for law enforcement officers. [0003] The traditional identification methods of meat products are mainly based on protein level analysis, including electrophoresis, immunoassay, ELISA and other techniques. In the process of operation, it is first necessary to prepare antibodies of the correspon...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6888C12Q2600/16
Inventor 于雷姜艳彬王海李颖蔡英华刘洪斌刘勇军王莹田亚平
Owner CHINA ANIMAL DISEASE CONTROL CENT