High density lipoprotein cholesterol detection kit

Abstract

The invention relates to a high density lipoprotein cholesterol detection kit which is a liquid-type dual-reagent composed of a reagent R1 and a reagent R2. The reagent R1 comprises: 0.5-4.0 mmol / L of a coupling agent (DSBmT), 3500-8500 U / L of a cholesterol oxidase (CHOD), 1000-4000 U / L of a peroxidase (POD), 0.5-1.5 g / L of a surfactant and 50-160 mmol / L of a buffer solution (pH=6.0). The reagent R2 comprises: 1.0-4.5 mmol / L of 4-aminoantipyrine (4-AAP), 700-1300 U / L of a cholesterol esterase, 0.5-1.5 g / L of a surfactant and 50-160 mmol / L of a buffer solution (pH=6.0). The high density lipoprotein cholesterol detection kit is better in specificity, has stronger in anti-interference capability, has anti-interference capabilities of hemoglobin, bilirubin, ascorbic acid and rntralipos and is higher in linearity. The kit is suitable for a full-automatic biochemical analyzer and has a great clinical application value. Stability time of the reagents can reach two years. The kit is reasonable in design, strong in practicality and is suitable for a wide application and popularization.

Description

technical field [0001] The invention relates to a high-density lipoprotein cholesterol detection kit, which belongs to the field of biological preparations. Background technique [0002] Lipids such as cholesterol cooperate with apoproteins in serum to form lipoproteins, which are also combined into the structure of lipoproteins, and form chylomicrons (CM), very low-density lipoproteins (VLDL), and low-density lipoproteins (LDL) , high-density lipoprotein (HDL) and other forms exist. A large-scale epidemiological survey in Frammingham in 1977 showed that the decrease of plasma high-density lipoprotein cholesterol (plasma HDL-C) was the main risk factor for ischemic heart disease. General attention. HDL is a preventive factor for heart disease caused by arteriosclerosis, so the determination of HDL-C has an important predictive role in the onset of arteriosclerosis, which is of great clinical significance. [0003] The direct method is divided into selective PEG modificat...

AI Technical Summary

Problems solved by technology

Although the enzyme modification method and PEG / antibody encapsulation method have many clinical applications, they are expensive, while the selective inhibition method has high precision, the linear range can reach 1500mg / L, and the recovery rate is 90%~110%, but in terms of anti-interference There are still defects

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