Composition having continuous anti-fatigue function
A composition and anti-fatigue technology, applied in the field of food science, can solve the problems of undiscovered mechanism and effect research reports, lack of energy, loss of cell replication ability, etc., so as to improve the energy overdraft of the human body, relieve the feeling of more fatigue, and restore high energy. Effects of Phosphate Levels
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Embodiment 1
[0037] Anti-fatigue test of embodiment 1 ribose combined with caffeine
[0038] 1 Establishment of fatigue mouse model
[0039] 1.1 Source of experimental animals
[0040] The experimental animals were ICR male mice, weighing 18-20 g, purchased from Beijing Weitong Lihua Experimental Animal Technology Co., Ltd., with the certificate number: SCXK (Beijing): 2012-0001.
[0041] 1.2 Mouse weight-bearing swimming test
[0042] Fix the lead wire equivalent to 7.5% of the mouse's body weight on the tail of the mouse, put it into a 30cm×25cm×25cm water tank, the water temperature is 30°C, the water depth is 20cm, 2 / box, swim until fatigue (sink into the water for 4 seconds), rest After 20 minutes, swim for the second time until fatigue, and record the time of swimming twice. Swim twice a day with weight in the morning and afternoon, and add up the two swimming times as one day's swimming time. The average swimming time of the previous two days is used as a reference. Continue to ...
Embodiment 2
[0050] Embodiment 2 Ribose and caffeine combined application ratio research
[0051] 1. Experimental animals
[0052] Experimental ICR male mice, weighing 18-20 g, are provided by Beijing Weitong Lihua Experimental Animal Technology Co., Ltd., and the certificate number is SCXK (Beijing): 2012-0001.
[0053] 2. Drugs and reagents
[0054] D-ribose: provided by Chengzhi Life Science and Technology Co., Ltd., batch number: 20121112.
[0055] Caffeine: provided by Chengzhi Life Technology Co., Ltd.
[0056] Positive drug: Cao Kaiyong men's nutrient solution, purchased from Tianjin Kaiyong Health Products Co., Ltd., batch number: 113002. The oral intake dose for humans is 10ml / bottle, three sticks a day, calculated on the basis of 60kg body weight, that is, 0.5ml / kg, and the dose for mice is 10 times that for humans, that is, 5ml / kg for mice.
[0057] 3. Experimental method
[0058] 3.1 Preparation of fatigue model
[0059] Fix the lead wire equivalent to 5% of the mouse’s b...
Embodiment 3
[0066] Embodiment 3 Ribose and caffeine restore the high-energy phosphate substance in the gastrocnemius muscle of fatigued mice
[0067] 1. Establishment of fatigue model
[0068] Fix the lead wire equivalent to 5% of the mouse’s body weight on the tail of the mouse, put it into a 40cm×50cm×25cm water tank, the water temperature is 30-33°C, the water depth is 20cm, 10 / box, swim until fatigue (submerged in the water for 3 seconds) , rest for 20 minutes and then swim for the second time until fatigue, then repeat the above steps after another 20 minutes of rest, record the time of each swim until the total time reaches 2 hours; repeat the morning experiment process in the afternoon until the total time reaches 2 hours.
[0069] 2. Grouping and administration
[0070] Immediately after modeling, the gastrocnemius muscles of 10 fatigued mice and 10 normal mice were taken, that is, the fatigue group and the normal control group; the remaining mice were randomly divided into 6 group...
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