A method for determining total flavonoid content in solution based on near-infrared spectroscopy

Abstract

The invention discloses a method for determining the content of total flavonoids in compound donkey-hide gelatin pulp based on near-infrared spectroscopy, which belongs to the field of traditional Chinese medicine research. The present invention prepares samples with different concentrations through concentration and dilution in the laboratory, forms a sample set together with the finished product samples of compound donkey-hide gelatin pulp, collects the near-infrared spectrum of the sample set, performs abnormal sample elimination and division of the sample set, and selects appropriate spectral bands, data pre-processing, etc. The processing method obtains the characteristic spectral information of the solution sample, uses the sodium nitrite-aluminum nitrate colorimetric method to measure the total flavonoid content as a reference value, and applies chemometrics technology to construct a quantitative calibration model for the relationship between the near-infrared spectrum of the sample and its total flavonoid content , collect the near-infrared spectrum of the finished product of compound donkey-hide gelatin paste to be tested, and use the constructed quantitative calibration model to quickly calculate its total flavonoid content. The method of the invention is beneficial to improving the quality control level of the finished product of the compound donkey-hide gelatin pulp, and ensures the stable and reliable quality of the finished product.

Description

technical field [0001] The invention relates to a method for determining the content of total flavonoids in a solution based on near-infrared spectroscopy, in particular to a method for determining the content of total flavonoids in compound donkey-hide gelatin pulp based on near-infrared spectroscopy, and belongs to the technical field of traditional Chinese medicine research. Background technique [0002] Finished drug inspection is the last quality control procedure that drugs must go through before entering the market, and it is directly related to the safety of consumers' medication. At present, the detection methods of traditional Chinese medicines are mostly chromatographic methods, which require complicated sample pretreatment before analysis, and the analysis time is also long, and the traditional determination method can only measure one index at a time, which prolongs the total consumption of the batch production process. Time. [0003] Compound donkey-hide gelat...

AI Technical Summary

Problems solved by technology

[0004] In the 2010 edition of "Chinese Pharmacopoeia", the determination of the content of compound donkey-hide gelatin pulp is only one item of total nitrogen, which is not enough to reflect the overall status of the active ingredients in the finished product of compound donkey-hide gelatin pulp, and it is difficult to meet the requirements for content analysis and monitoring of the finished product of compound donkey-hide gelatin pulp

[0006] However, due to the complex composition of Chinese patent medicines, the low content of active ingredients and the serious absorption overlap in their near-infrared spectra, there are still few reports on the near-infrared spectroscopy of traditional Chinese medicine preparations.

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