Cutting seedling culture method of akebia trifoliate

Abstract

The invention relates to a cutting seedling culture method of akebia trifoliate, which is characterized by comprising a cutting branch maternal plant culture phase and a cutting branch seedbed culture phase. The method has the benefits that through the adoption of the cutting seedling culture method of the akebia trifoliate, (1), the frequency of callus induction can reach 98.6-99.8%; (2), the differentiation rate of root primordia can reach 98.5-99.5%; (3), the rooting percentage can reach 97.9-98.6%; (4), the survival rate can reach 96.7-97.8%; (5), the seedling rate can reach 96.4-96.7%; (6), the maternal plant stipe utilization rate can reach 90-93%.

Description

technical field [0001] The invention relates to a cutting seedling raising method of Akebia trifoliate. Background technique [0002] Akebia trifoliate is a deciduous woody vine, with three compound leaves, leaflets oval, blunt or short pointed at the apex, rounded at the base, sometimes slightly heart-shaped, with lobed or wavy edges, slender petioles, open in spring and summer Purple-red flowers, dioecious. [0003] The propagation methods of Akebia clover mainly include several main methods such as seed propagation, layering propagation, branch propagation, and cutting propagation. Seed propagation is simple and easy, and the bred seedlings bear fruit late, usually after 3 years, and are generally not used in production; layering propagation generally blooms and bears fruit in the second year after planting, but the layering operation occupies a large area and is not easy to operate. It is convenient but difficult to implement; although multiple plants can be propagated...

AI Technical Summary

Problems solved by technology

Seed propagation is simple and easy, and the bred seedlings bear fruit late, usually after 3 years, and are generally not used in production; layering propagation generally blooms and bears fruit in the second year after planting, but the layering operation occupies a large area and is not easy to operate. It is convenient but difficult to implement; although multiple plants can be propagated in one bag, the propagation coefficient is small, and it is only suitable for ex-situ conservation of germplasm resources and the initial construction stage of female parent gardens; cutting propagation can be carried out all year round, and cuttings in spring Mainly, with a high reproduction coefficient, it is the main method for the propagation of Akebia trifoliate seedlings, and is suitable for large-scale production and supply of high-quality seedlings

[0004] The cutting survival rate of Akebia trifoliata is generally 50-90%, and the seedling rate is 30-60%. The range of variation is large, and the reproducibility of the results is not strong.

The main reason is that researchers do not really understand the interaction of many factors that affect the rooting and germination of cuttings, including the nutritional status and age of the cuttings' mother plants, the collection and processing of cuttings, the season of cuttings, and the soil, water and fertilizer, light, environmental factors, etc.

There are many research reports on cutting propagation methods of Akebia clover seedlings, which are similar with minor differences, mostly based on the comparative analysis of a certain (or group) test results. No breakthrough has been made in technology, and no feasible Akebia trifoliata has been formed. Seedling raising method by cutting

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