A kind of Corynebacterium beijing microbial adsorbent and preparation method thereof
A Beijing corynebacterium and microbial adsorption technology, applied in chemical instruments and methods, adsorption water/sewage treatment, other chemical processes, etc., can solve the problems of limited application of adsorption method, high price, etc., and achieve low requirements and cost-effective advantages , the effect of convenient operation
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Embodiment 1
[0035] Microorganisms of Corynebacterium Beijing loaded on polyurethane foam
[0036] Corynebacterium Beijing was selected as the microorganism, which is solid waste used for amino acid synthesis and provided by the biological laboratory. Put the sterilized Corynebacterium Beijing into a centrifuge for centrifugation, take it out and put it in an oven, dry it at 60°C, and grind it into powder with a mortar. A cube block of 10 mm x 10 mm x 10 mm was processed from recycled polyurethane foam. Take 20 samples of blank polyurethane foam, polyurethane foam soaked in 95% ethanol for 5 minutes, and polyurethane foam modified with silane coupling agent KH-570. Before loading microorganisms, first weigh the samples and record their original weights. Then put it into a 250mL Erlenmeyer flask, add 3g of Corynebacterium Beijing, put it on a shaker, shake it continuously for 1 hour, put it in a tray, put it in an oven at 80°C and dry it, measure its weight, and calculate the load rate . ...
Embodiment 2
[0040] Adhesion of Corynebacterium Beijing with Polyurethane Foam and Polyvinyl Alcohol
[0041] Put the sterilized Corynebacterium Beijing into a centrifuge for centrifugation, take it out and put it in an oven, dry it at 60°C, and grind it into powder with a mortar. Weigh 2g of polyvinyl alcohol, put it into a small beaker, add 98g of distilled water, stir and heat at 90°C until completely dissolved, and prepare a 2wt% glue solution. Take 20 small cubes of 10mm×10mm×10mm blank polyurethane foam, put them into the prepared polyvinyl alcohol glue, place them on a vibrator to oscillate, and soak for 3 hours. Then take out the polyurethane foam, press dry the glue held by the foam, and do not use an oven to dry it, because it is necessary to maintain a layer of glue film on the network structure of the polyurethane foam, so that the Corynebacterium Beijing can be bonded. When loading Corynebacterium Beijing, the air blowing method was used. The prepared polyurethane plus polyv...
Embodiment 3
[0043] Adhesion of Corynebacterium Beijing to Polyurethane Foam Added with Polyvinyl Alcohol Modified by Silane Coupling Agent KH-570
[0044] Put the sterilized Corynebacterium Beijing into a centrifuge for centrifugation, take it out and put it in an oven, dry it at 60°C, and grind it into powder with a mortar. Weigh 2g of polyvinyl alcohol, put it into a small beaker, add 98g of distilled water, stir and heat at 90°C until completely dissolved, and prepare a 2wt% glue solution. Take 30 small cubes of polyurethane foam modified by silane coupling agent, 10mm×10mm×10mm, put them into the prepared polyvinyl alcohol glue, place them on an oscillator to vibrate, and soak for 3 hours. Then take out the polyurethane foam, press dry the glue held by the foam, do not use an oven to dry, because it is necessary to maintain a layer of glue film on the network structure of the polyurethane foam, so that microorganisms can bond. When carrying microorganisms, the air blowing method is u...
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