Serum-free CHO cell culture medium and preparation method thereof
A serum-free culture medium and a culture medium technology, applied in the biological field, can solve problems such as unfavorable separation and purification, high price, pollution, etc., and achieve the effects of avoiding transportation risks, reducing transportation costs, and avoiding unfavorable factors.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
preparation example Construction
[0068] A preparation method of CHO cell serum-free medium, wherein, said CHO cell serum-free medium also can be made into powder medium, comprising the following steps:
[0069] Step 1. Weigh each component in the CHO cell serum-free medium according to the formula and mix them uniformly;
[0070] Step 2, fully grind, grind to 100-200 mesh sieve;
[0071] Step 3, high-temperature sterilization, the present invention adopts a high-pressure steam damp heat sterilization method, the sterilization temperature is 126-132°C, the steam pressure is 0.044-0.049MPa, and the sterilization time is 3-7min.
[0072] Step 4, aseptic dispensing, a certain dose of dispensing and sealing is carried out by aseptic operation.
[0073] It can be prepared by dissolving the powdered CHO cell serum-free medium in pyrogen-free sterile water according to the formula quantity.
[0074] The full name of the CHO cells is Chinese hamster ovary cells. The CHO cell serum-free medium is a medium formula des...
Embodiment 1
[0086] Weigh each component according to the following formula, and all raw materials of the present invention are purchased from Sigma cell culture grade raw materials, and stored according to relevant requirements.
[0087] Composition and dosage of L-amino acid:
[0088] components
Dosage (unit: mg / kg)
Alanine
10.26
arginine
155.82
Asparagine
247.32
cysteine
38.26
glutamic acid
8.16
Glycine
8.16
Histidine
32.50
Isoleucine
131.25
Leucine
165.23
Lysine
190.26
Methionine
60.42
Phenylalanine
83.56
proline
35.35
serine
92.15
threonine
95.24
Tryptophan
51.41
Tyrosine
142.13
[0089] Valine
112.31
Glutamine
1248.93
[0090] Composition and dosage of vitamins:
[0091] components
Dosage (unit: mg / kg)
Vitamin B6
0.2
...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap