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Method for detecting damage of mitochondria in prostate gland cells

A detection method, prostate technology, applied in the detection field, can solve the problems of expensive equipment, complicated operation, difficult implementation, etc., and achieve the effect of low cost

Active Publication Date: 2014-10-01
青岛宏利源高新技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In order to solve the problems of complex operation, expensive equipment, and difficult implementation of the method for detecting mitochondrial damage in prostate gland cells in the prior art, the present invention provides a method for detecting free ferrous protoporphyrin in prostate fluid cells, and then understanding the mitochondrial damage of prostate gland cells. The method of whether there is damage, the method provided by the invention is simple and easy, with low cost, and can quickly detect the mitochondrial damage of prostate gland cells

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] 1) Obtain prostatic fluid 1-2;

[0015] 2) Put the prostatic fluid into the sample bottle, which is pre-installed with acetic acid buffer solution with a pH value of 4.5, the amount of the buffer solution is 2ml, mix the prostatic fluid with the sample preservation solution, and obtain the sample solution;

[0016] 3) Add 1ml of reagent A and 1ml of reagent B to the sample bottle in sequence; mix well, observe the color change within 2 minutes, and compare the test results with the color guide;

[0017] The reagent A is bis(4.dimethylaminophenyl)methane, which is dissolved in methanol to prepare a solution; preferably, the concentration of the bis(4.dimethylaminophenyl)methane solution is 0.002mol / L;

[0018] The reagent B is 1.5% hydrogen peroxide solution.

[0019]

[0020] 【judgement standard】

[0021] The liquid in the sample bottle has no color or is slightly blue (1) Negative

[0022] blue (+) positive

[0023] dark blue (++) strongly positive

[0024]...

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Abstract

The invention discloses a method for detecting damage of mitochondria in prostate gland cells. The method comprises the following detection steps: extracting 1-2ml of prostatic fluid, adding the prostatic fluid into a sample bottle filled with a buffer solution, and sequentially adding 1ml of a reagent A and 1ml of a reagent B into the sample bottle; and uniformly mixing, observing color change in 2 minutes, comparing the color change with a colorimetric plate, thus obtaining a detection result. The method is simple, practical, quick and high in sensitivity.

Description

technical field [0001] The invention relates to a detection method, in particular to a detection method for mitochondrial damage of prostate gland cells. Background technique [0002] Mitochondria are the most important organelles in human cells and the "power factories" of cells. The detection of mitochondria in damaged prostate epithelial cells includes the detection of mitochondrial permeability transition pore (mitochon2dria permeability transition pore, MPTP), the detection of membrane phospholipids, the detection of membrane potential, the detection of 2mtDNA damage, the detection of intracellular calcium ion concentration, etc. This type of detection operation is very complicated and requires sophisticated instruments and equipment, which is difficult to complete in general laboratories, which greatly limits the detection of mitochondrial damage in prostate gland cells. Before the above difficult detection, use a simple and low-cost method to conduct a preliminary d...

Claims

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Application Information

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IPC IPC(8): G01N21/78
Inventor 温鑫崔子伦
Owner 青岛宏利源高新技术有限公司
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