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Epidermal tissue culturing method, and prepared epidermal tissue culture and application thereof

A technology of epidermal tissue and culture method, applied in the field of cell culture, which can solve the problems of difficult and uneven distribution of pigment, and achieve the effect of easy transplantation, high adhesion rate and good prognosis

Inactive Publication Date: 2014-10-15
瑞田投资有限公司 +1
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  • Summary
  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Compared with autologous skin extraction, this therapy can treat a relatively large area, but it causes uneven distribution of pigment at the injection site, and it is very difficult to achieve uniform distribution of pigment in large area treatment

Method used

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  • Epidermal tissue culturing method, and prepared epidermal tissue culture and application thereof
  • Epidermal tissue culturing method, and prepared epidermal tissue culture and application thereof
  • Epidermal tissue culturing method, and prepared epidermal tissue culture and application thereof

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Embodiment Construction

[0039] Specific implementation plan

[0040] The following are examples and specific descriptions of the epidermal tissue culture, use and manufacturing method of the present invention, but the present invention is not limited thereto.

[0041] (1) Skin sample collection and production of epidermal tissue culture:

[0042] A 70% alcohol cotton ball was used for disinfection on the waist of the subject, and tissue pieces were collected from the patient's body. The collected tissue pieces were immersed in iodine solution for disinfection, and then washed with PBS. Cut the tissue pieces into small pieces, immerse them in TryPLE (Gibco) diluted 5 times in DMEM, and leave them at 4°C for 16-24 hours. Stir at room temperature for 30 minutes, and remove the residue using a cell strainer (BD company). The formed cell suspension is centrifuged to recover the cells. Then, the residue was treated with collagenase at 37°C for 3 hours, and then the cells were recovered in the same manner as d...

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Abstract

The invention relates to an epidermal tissue culturing method, an epidermal tissue culture prepared by using the method and application of the epidermal tissue culture in the field of cosmetic dermatology. The epidermal tissue culturing method comprises a step of culturing a culturing mixture of melanin cells and epidermal keratinocytes under the conditions that the quantity of the epidermal keratinocytes is 100 and the quantity of the melanin cells is in a range of 0.01 to 50.

Description

Technical field [0001] The invention belongs to the field of cell culture, and specifically relates to an epidermal tissue culture method, an epidermal tissue culture obtained by the method and the application of the epidermal tissue culture in the field of skin beauty. Background technique [0002] Epidermal tissue cultures have a wide range of applications in the medical and cosmetic fields. For example, epidermal tissue cultured from epidermal cells and dermal cells can be used to treat burns. This treatment can also be used for some scars. After a large area of ​​moles is removed, cultured epidermal tissue is usually attached to treat the moles without leaving scars. [0003] The most important factor that determines skin color is a pigmented substance called melanin. The formation of melanin is produced by melanocytes that exist in the basal layer of the epidermis. The acquired vitiligo vulgaris of depigmentation is caused by abnormal obstruction of the formation of melani...

Claims

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Application Information

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IPC IPC(8): C12N5/071A61L27/38A61L27/60
Inventor 宇山太郎吴复跃大槻忠男何振东
Owner 瑞田投资有限公司
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