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A method for measuring DNA hybridization using graphene-modified high electron mobility transistors

A high electron mobility, transistor technology, applied in the field of transfer technology and DNA immobilization, can solve the problem of no detection line

Active Publication Date: 2016-08-03
UNIV OF SCI & TECH BEIJING
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The work of Kang et al. requires a complex chemical process to immobilize DNA on the HEMT surface, and they did not mention the detection line and sensitivity of DNA hybridization with HEMT in the article

Method used

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  • A method for measuring DNA hybridization using graphene-modified high electron mobility transistors
  • A method for measuring DNA hybridization using graphene-modified high electron mobility transistors
  • A method for measuring DNA hybridization using graphene-modified high electron mobility transistors

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] HEMTs with dimensions of 2 mm x 5 mm were constructed.

[0021] Use 1 mg / mL, 5 μL graphene ethanol dispersion and 23 base pair probe DNA solution (0.1 μM, 20 μL) to incubate the mixture for 30 minutes at a temperature of 18 ° C, and add the mixture dropwise to the HEMT gate Dry the polar surface at 4°C for 12 hours before performing DNA hybridization detection.

[0022] The DNA sequence used in this embodiment is as follows:

[0023] Probe DNA: 5'-AAT-CAA-CTG-GGA-GAA-TGT-AAC-TG-3'

[0024] Target DNA: 5'-CAG-TTA-CAT-TCT-CCC-AGT-TGA-TT-3'

[0025] Arbitrary DNA: 5'-ACC-TTC-CTC-CGC-AAT-ACT-CCC-3'

[0026] DNA hybridization detection is performed on the HEMT that has immobilized the probe DNA, and the "two-step mode" DNA hybridization response current can be obtained in about 5 minutes. If any DNA is dropped onto the surface of the HEMT gate, only a "one step mode" response current can be obtained. A novel current recognition mode for graphene-modified HEMTs to detect...

Embodiment 2

[0029] A HEMT of size 5mm x 5mm was constructed.

[0030] Use 0.5 mg / mL, 5 μL graphene ethanol dispersion and 27 base pair probe DNA solution (0.1 μM, 25 μL) to incubate the mixture for 40 minutes at a temperature of 20 ° C, and add the mixture dropwise to the HEMT Dry the surface of the gate at 2°C for 12 hours before performing DNA hybridization detection.

[0031] The DNA sequence used in this embodiment is as follows:

[0032] Probe DNA: 5'-AAT-CAA-CTG-GGA-GAA-TGT-AAC-TGA-CCT-3'

[0033] Target DNA: 5'-AGG-TCA-GTT-ACA-TTC-TCC-CAG-TTG-ATT-3'

[0034] Arbitrary DNA: 5'-ACC-TTC-CTC-CGC-AAT-ACT-CCC-ACT-CTG-3'

[0035] DNA hybridization detection is performed on the HEMT that has immobilized the probe DNA, and the "two-step mode" DNA hybridization response current can be obtained in about 5 minutes. If any DNA is dropped onto the surface of the HEMT gate, only a "one step mode" response current can be obtained.

Embodiment 3

[0037] HEMTs with dimensions of 2mm x 2mm were constructed.

[0038] Use 1 mg / mL, 10 μL graphene ethanol dispersion and 24 base pair probe DNA solution (0.2 μM, 30 μL) to incubate the mixture for 50 minutes at a temperature of 22 ° C, and add the mixture dropwise to the HEMT gate The pole surface was dried at 0°C for 12 hours before DNA hybridization detection.

[0039] The DNA sequence used in this embodiment is as follows:

[0040] Probe DNA: 5'-AAT-CAA-CTG-GGA-GAA-TGT-AAC-TGA-3'

[0041] Target DNA: 5'-TCA-GTT-ACA-TTC-TCC-CAG-TTG-ATT-3'

[0042] Arbitrary DNA: 5'-TTC-CTC-CGC-AAT-ACT-CCC-ACT-CTG-3'

[0043] DNA hybridization detection is performed on the HEMT that has immobilized the probe DNA, and the "two-step mode" DNA hybridization response current can be obtained in about 5 minutes. If any DNA is dropped onto the surface of the HEMT gate, only a "one step mode" response current can be obtained.

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Abstract

The invention relates to a hybridization method for measuring DNA by modifying a high electron mobility transistor with graphene, using graphene to modify a high electron mobility transistor and immobilizing probe DNA to measure DNA hybridization, so as to realize a fast, sensitive and novel current response mode A method for measuring DNA hybridization using graphene-modified high-electron-mobility transistors for DNA hybridization detection. The method first uses molecular beam epitaxy to construct high-electron-mobility transistors; then graphene is used to immobilize probe DNA to HEMT gates Surface; finally drop the target DNA for hybridization measurement to obtain the response current. Using this method, the DNA hybridization detection of actual samples can be realized. The method simplifies the process of immobilizing the probe DNA on the surface of the device, and discards the complicated chemical process. Novel DNA hybridization recognition patterns were obtained in this method.

Description

technical field [0001] The invention relates to graphene transfer technology and DNA immobilization technology. Graphene is used to modify high electron mobility transistor (HEMT) and probe DNA is fixed to measure DNA hybridization and realize fast, sensitive and novel current response mode of DNA. Hybridization detection. Background technique [0002] Realizing the rapid and sensitive detection of DNA hybridization plays a very important role in food safety detection, environmental monitoring, disease diagnosis, criminal investigation and so on. At present, there are mainly electrochemical methods and photochemical methods for DNA detection. Although the above methods have obtained good detection results, there are still problems of low detection efficiency and high detection cost. Using field effect transistor (FET) to detect DNA hybridization is an effective means to solve the above problems. High electron mobility transistors (HEMTs), as a special kind of FET, have gre...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/26
Inventor 张跃章潇慧廖庆亮刘硕王钦玉张铮
Owner UNIV OF SCI & TECH BEIJING
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