Method for inducing retinal and choroidal neovascularization by AAV-VEGF (adeno-associated virus transduced-vascular endothelial growth factor)
An AAV-VEGF, new blood vessel technology, applied in the field of AAV-VEGF-induced retinal and choroidal neovascularization, can solve the problem of inconclusive complement components and so on
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[0009] The following will clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only some of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.
[0010] A method for inducing retinal and choroidal neovascularization by adeno-associated virus-transduced vascular endothelial growth factor (AAV-VEGF) is provided, and the specific operation process is as follows:
[0011] Instill the mydriatic agent compound tropicamide eye drops in the conjunctival sac of the animal until the pupil is completely dilated. Intramuscularly inject ketamine (10 mg / kg), and anesthetize the animal with sodium pentobarbital (20 mg / kg) intravenously until the corneal reflex and pain refle...
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