Method for inducing retinal and choroidal neovascularization by AAV-VEGF (adeno-associated virus transduced-vascular endothelial growth factor)

An AAV-VEGF, new blood vessel technology, applied in the field of AAV-VEGF-induced retinal and choroidal neovascularization, can solve the problem of inconclusive complement components and so on

Inactive Publication Date: 2014-10-22
JOINN LAB (SUZHOU) INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, the participation of inflammation and complement pathways has been confirmed in some retinal neovascular diseases, but the specific process and the involved complement components are still inconclusive

Method used

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Embodiment Construction

[0009] The following will clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only some of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0010] A method for inducing retinal and choroidal neovascularization by adeno-associated virus-transduced vascular endothelial growth factor (AAV-VEGF) is provided, and the specific operation process is as follows:

[0011] Instill the mydriatic agent compound tropicamide eye drops in the conjunctival sac of the animal until the pupil is completely dilated. Intramuscularly inject ketamine (10 mg / kg), and anesthetize the animal with sodium pentobarbital (20 mg / kg) intravenously until the corneal reflex and pain refle...

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Abstract

The invention discloses a method for inducing retinal and choroidal neovascularization by AAV-VEGF (adeno-associated virus transduced-vascular endothelial growth factor), and the AAV-VEGF is applied to the animal body by a subretinal injection method. Through the way, according to the method for inducing the retinal and choroidal neovascularization by the AAV-VEGF, by subretinal injection of AAV-VEGF gene, retinal neovascularization hyperplasia can be promoted, meanwhile the retinal neovascularization induced by the AAV-VEGF can be maintained for a long term, the successful rate can reach over 98%, and diabetic fundus lesions or wet age-related maculopathy simulated retinopathy can be obtained.

Description

technical field [0001] The invention relates to the field of medical technology, in particular to a method for inducing retinal and choroidal neovascularization by AAV-VEGF. Background technique [0002] Retinal neovascular disease refers to blinding vitreoretinal disease caused by pathological changes such as hemorrhage, exudation, and proliferation of new blood vessels. It is one of the most serious blinding eye diseases in my country and the world, including diabetic retinopathy, Retinal vascular occlusive disease, retinal periphlebitis, and retinopathy of prematurity are increasingly threatening the visual health of the Chinese population. For a long time, there have been different opinions on the mechanism of retinal neovascularization, such as the role of vasoactive factors and endogenous angiogenesis inhibitors in retinal neovascularization. They do not act alone and independently in the eye, but interact with multiple factors Coordinate and jointly regulate angiogene...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/18
Inventor 梅明左丛林冯宇霞
Owner JOINN LAB (SUZHOU) INC
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