Specific primer for identifying bungarus parvus based on DNA barcodes, PCR method and kit

Abstract

The invention belongs to the technical field of traditional Chinese medicine and Chinese herbal medicine identification and relates to a specific primer for identifying bungarus parvus based on DNA barcodes, a PCR method and a kit, which is used for identifying the authenticity of the bungarus parvus. The method comprises the following steps: extracting DNA, performing specific PCR amplification, and performing electrophoresis detection. The specific primer is simple, convenient and quick, high in specificity, good in commonality and the like. The specific primer is used for identifying the bungarus parvus and medicinal materials, and is especially suitable for quick identification of processed drug preparations containing the bungarus parvus due to relatively short target fragments.

Description

technical field [0001] The invention belongs to the technical field of identification of traditional Chinese medicines and medicinal materials, and relates to a high-specificity primer, a PCR method and a kit that can specifically identify white snakes. Background technique [0002] The golden snake is the dried body of Bungarus multicinctus Blyth juvenile snake of Cobra family. It is recorded in the 2010 edition of "Chinese Pharmacopoeia". It has the effects of expelling wind, dredging collaterals and relieving spasm. The application history of the golden snake is relatively short, but its medicinal effect is remarkable. Hemiplegia, convulsions, tetanus, leprosy, mange. [0003] Due to the limitation of its wild resource scale and natural regeneration capacity, and the impact of overhunting in recent decades, the production capacity of the golden snake has dropped sharply, resulting in its high market price. Driven by the shortage of supply and the maximization of inter...

AI Technical Summary

Problems solved by technology

However, Wang Yiquan and Feng Chengqiang reported specific primers aimed at the design of Cytb sequences to identify white snakes. The types of counterfeit species reported were less (5 kinds), and there were 9 kinds of counterfeit snakes found in reports, so their research objects not comprehensive

The target fragment reported in the CN101613759A patent is relatively long (about 560bp). Since the DNA is prone to degradation during processing or after long-term storage, the longer target fragment is relatively difficult to amplify. Therefore, this method still has potential defects

However, DNA barcoding technology needs to determine the gene sequence of the species, and the operation process is relatively cumbersome.

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