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A method for increasing the yield of fatty acid in microalgae under heterotrophic conditions

A fatty acid and microalgae technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problem of low yield of fatty acid in microalgae

Active Publication Date: 2017-05-10
UNIV OF SCI & TECH OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to solve the technical problem that the yield of fatty acid in microalgae is not high in the prior art, the purpose of the present invention is to provide a microalgae method for improving the yield of fatty acid in heterotrophic

Method used

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  • A method for increasing the yield of fatty acid in microalgae under heterotrophic conditions
  • A method for increasing the yield of fatty acid in microalgae under heterotrophic conditions
  • A method for increasing the yield of fatty acid in microalgae under heterotrophic conditions

Examples

Experimental program
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Effect test

Embodiment 1

[0068] Example 1 Study on Phosphorus Limitation on Fatty Acid Content and Yield of Heterotrophic Chlorella Under Nitrogen Conditions

[0069] The composition of the autotrophic medium is as follows: NaNO 3 1500mg / L, K 2 HPO 4 ·3H 2 O40mg / L, MgSO 4 ·7H 2 O75mg / L, Na 2 CO 3 20mg / L, CaCl 2 27mg / L, citric acid monohydrate 6mg / L, ferric ammonium citrate 6mg / L, Na 2 EDTA1mg / L, 1mL trace metal solution (※ trace metal solution: H 3 BO 3 2.86mg / L, MnCl 2 4H 2 O1.81mg / L, ZnSO 4 ·7H 2 O0.222mg / L, CuSO 4 ·5H 2 O0.079mg / L, CoCl 2 ·6H 2 O0.050mg / L, Na 2 MoO 4 2H 2 (00.39mg / L)

[0070] The composition of the heterotrophic medium under the condition of nitrogen deficiency is: glucose 10g / L, NaNO 3 1500mg / L, K 2 HPO 4 ·3H 2 O0~258mg / L, MgSO 4 ·7H 2 O75mg / L, Na 2 CO 3 20mg / L, CaCl 2 27mg / L, citric acid monohydrate 6mg / L, ferric ammonium citrate 6mg / L, Na 2 EDTA1mg / L, 1mL trace metal solution (※ trace metal solution: H 3 BO 3 2.86mg / L, MnCl 2 4H 2 O1.81mg / L, Zn...

Embodiment 2

[0075] Example 2 Study on Phosphorus Limitation on Fatty Acid Content and Yield of Heterotrophic Chlorella under Nitrogen Deficiency Conditions

[0076] The composition of the autotrophic medium is as follows: NaNO 3 1500mg / L, K 2 HPO 4 ·3H 2 O40mg / L, MgSO 4 ·7H 2 O75mg / L, Na 2 CO 3 20mg / L, CaCl 2 27mg / L, citric acid monohydrate 6mg / L, ferric ammonium citrate 6mg / L, Na 2 EDTA1mg / L, 1mL trace metal solution (※ trace metal solution: H 3 BO 3 2.86mg / L, MnCl 2 4H 2 O1.81mg / L, ZnSO 4 ·7H 2 O0.222mg / L, CuSO 4 ·5H 2 O0.079mg / L, CoCl 2 ·6H 2 O0.050mg / L, Na 2 MoO4·2H 2 (00.39mg / L)

[0077] The composition of the heterotrophic medium under the condition of nitrogen deficiency is: glucose 10g / L, K 2 HPO 4 ·3H 2 O0~258mg / L, MgSO 4 ·7H 2 O75mg / L, Na 2 CO 3 20mg / L, CaCl 2 27mg / L, citric acid monohydrate 6mg / L, ferric ammonium citrate 6mg / L, Na 2 EDTA1mg / L, 1mL trace metal solution (※ trace metal solution: H 3 BO 3 2.86mg / L, MnCl 2 4H 2 O1.81mg / L, ZnSO 4 ·7H...

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Abstract

A method for increasing yield of microalgae fatty acid in heterotrophism. The method comprises following steps: a. performing enlarge cultivation to microalgae in an autotrophic culture medium to obtain enough microalgae seeds; b. inoculating the microalgae seeds to a phosphorus-limited heterotrophic culture medium; and c. performing phosphorus-limited heterotrophic cultivation.

Description

technical field [0001] The invention belongs to the field of microalgae biotechnology, and relates to a method for improving the yield of microalgae fatty acid under heterotrophy. Background technique [0002] Biodiesel, namely fatty acid methyl ester, is a kind of biomass energy with renewable characteristics. It is mainly derived from oil-producing crops, such as soybeans and sunflower seeds, but its further development is limited by shortcomings such as low yield, high cost, and competition for land with other crops. A portion of microalgae is rich in oil and has become a new source of biodiesel due to its short growth cycle and no need to occupy arable land. [0003] Nowadays, most of the research on oil production of microalgae adopts the photoautotrophic culture method. However, due to the high requirement of light for photoautotrophic culture, resulting in low cell density, heterotrophic culture can overcome this shortcoming. Heterotrophic culture does not require ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/64C12R1/89
Inventor 曾建雄申晓菲夏修阳储菲菲余世金胡昊
Owner UNIV OF SCI & TECH OF CHINA
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