Method for improving yield of heterotrophic microalgal fatty acid with sodium acetate

A sodium acetate and fatty acid technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problem of low yield of fatty acid in microalgae

Inactive Publication Date: 2015-04-01
UNIV OF SCI & TECH OF CHINA
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0006] In order to solve the technical problem that the yield of fatty acid in microalgae is not high in the prior art, the purpose of the present invention is to provide a microalgae method for improving the yield of fatty acid in heterotrophic

Method used

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  • Method for improving yield of heterotrophic microalgal fatty acid with sodium acetate
  • Method for improving yield of heterotrophic microalgal fatty acid with sodium acetate
  • Method for improving yield of heterotrophic microalgal fatty acid with sodium acetate

Examples

Experimental program
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Effect test

Embodiment 1

[0071] Example 1 Study on Nitrogen Deficiency Conditions on Fatty Acid Content and Yield of Heterotrophic Chlorella

[0072] The composition of the autotrophic medium is as follows: NaNO 3 1500mg / L, K 2 HPO 4 ·3H 2 O 20mg / L, MgSO 4 ·7H 2 O 75mg / L, Na 2 CO 3 20mg / L, CaCl 2 27mg / L, citric acid monohydrate 6mg / L, ferric ammonium citrate 6mg / L, Na 2 EDTA 1mg / L, 1mL trace metal solution (※ trace metal solution: H 3 BO 3 2.86mg / L, MnCl 2 4H 2 O 1.81mg / L, ZnSO 4 ·7H 2 O 0.222mg / L, CuSO 4 ·5H 2 O 0.079mg / L, CoCl 2 ·6H 2 O 0.050mg / L, Na 2 MoO 4 2H 2 O 0.39mg / L)

[0073] The composition of the heterotrophic medium under the condition of nitrogen deficiency is: sodium acetate 5g / L, K 2 HPO 4 ·3H 2 O 15mg / L, MgSO 4 ·7H 2 O 75mg / L, Na 2 CO 3 20mg / L, CaCl 2 27mg / L, citric acid monohydrate 6mg / L, ferric ammonium citrate 6mg / L, Na 2 EDTA 1mg / L, 1mL trace metal solution (※ trace metal solution: H 3 BO 3 2.86mg / L, MnCl 2 4H 2 O 1.81mg / L, ZnSO 4 ·7H 2 O 0.222m...

Embodiment 2

[0078] Example 2 Study on Nitrogen Deficiency Conditions on Fatty Acid Content and Yield of Heterotrophic Scenedesmus

[0079] The composition of the autotrophic medium is as follows: NaNO 3 1500mg / L, K 2 HPO 4 ·3H 2 O 20mg / L, MgSO 4 ·7H 2 O 75mg / L, Na 2 CO 3 20mg / L, CaCl 2 27mg / L, citric acid monohydrate 6mg / L, ferric ammonium citrate 6mg / L, Na 2 EDTA 1mg / L, 1mL trace metal solution (※ trace metal solution: H 3 BO 3 2.86mg / L, MnCl 2 4H 2 O 1.81mg / L, ZnSO 4 ·7H 2 O 0.222mg / L, CuSO 4 ·5H 2 O 0.079mg / L, CoCl 2 ·6H 2 O 0.050mg / L, Na 2 MoO 4 2H 2 O 0.39mg / L)

[0080] The composition of the heterotrophic medium under the condition of nitrogen deficiency is: sodium acetate 5g / L, K 2 HPO 4 ·3H 2 O 15mg / L, MgSO 4 ·7H 2 O 75mg / L, Na 2 CO 3 20mg / L, CaCl 2 27mg / L, citric acid monohydrate 6mg / L, ferric ammonium citrate 6mg / L, Na 2 EDTA 1mg / L, 1mL trace metal solution (※ trace metal solution: H 3 BO 3 2.86mg / L, MnCl 2 4H 2 O 1.81mg / L, ZnSO 4 ·7H 2 O 0.22...

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Abstract

The invention relates to a method for improving yield of heterotrophic microalgal fatty acid with sodium acetate. The method comprises the following steps: a) performing amplified culture on microalage in an autotrophic culture medium to obtain sufficient algae; b) inoculating the algae in the step a) into a nitrogen-deficiency heterotrophic culture medium; and c) performing nitrogen-deficiency heterotrophic culture.

Description

technical field [0001] The invention belongs to the field of microalgae biotechnology, and relates to a method for increasing the fatty acid yield of heterotrophic microalgae with sodium acetate. Background technique [0002] Biodiesel is a renewable biomass energy with excellent environmental performance and combustion performance. Traditional biodiesel is derived from crops such as corn and soybeans, but the yield is low, the cost is high, and there is a hidden danger of "competing with food for land". Therefore, it is urgent to find a new raw material for biomass energy. Microalgae have the advantages of wide distribution, short growth cycle, and high oil content, so microalgae are likely to become the most important source of biomass energy in the future. [0003] Nowadays, most of the research on oil production of microalgae focuses on the photoautotrophic culture method. However, because the photoautotrophic culture method has high requirements on light, its cost is ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/64C12R1/89
CPCC12P7/6409
Inventor 曾建雄刘静静申晓菲
Owner UNIV OF SCI & TECH OF CHINA
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