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Antagonistic phytopathogen strain and application thereof

A technology of phytopathogenic bacteria and phytopathogenic fungi, applied in the field of microorganisms, can solve problems such as environmental pollution and difficult chemical pesticide control, and achieve significant effects, strong inhibitory effects, and growth-inhibiting effects

Active Publication Date: 2014-11-26
北京百瑞盛田环保科技发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The antagonistic bacterial strain Bacillus subtilis ZSH-1 isolated and screened from poplar root soil in the present invention has high-efficiency antagonistic effects on various plant pathogenic fungi and can also produce volatile antibacterial substances, and is a broad-spectrum biocontrol bacterial strain. The application of the bacterial strain of the antagonistic bacteria Bacillus subtilis ZSH-1 of the present invention solves the current problems of difficult chemical pesticide control of plant diseases and serious environmental pollution

Method used

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  • Antagonistic phytopathogen strain and application thereof
  • Antagonistic phytopathogen strain and application thereof
  • Antagonistic phytopathogen strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Example 1 Isolation, screening and identification of Bacillus subtilis ZSH-1

[0049] 1. Isolation and screening of Bacillus subtilis ZSH-1

[0050] The bacterial strain of the present invention is collected from the poplar experimental field soil of the Forest Protection Experimental Station of Beijing Forestry University in Haidian District, Beijing.

[0051] 1A) Take 5 g of poplar root soil, put it into a sterilized Erlenmeyer flask equipped with 45 mL of sterile water, place it on a shaker, vibrate at 180 rpm for 30 min at 28° C. -1 Concentration of the soil dilution); then use 10-fold gradient dilution method to dilute successively to obtain 10 -3 、10 -4 、10 -5 Concentration gradient dilution, take 0.1mL from each concentration gradient suspension, and spread it on the LB medium plate with a sterile applicator, and use sterile water as a control, spread it evenly and put it in a constant temperature box at 28°C Inverted culture; each treatment setting level rep...

Embodiment 2

[0073] Example 2 Exploration of the biological characteristics of Bacillus subtilis ZSH-1

[0074] 1A) Pick a circle of activated Bacillus subtilis ZSH-1 strain and inoculate it in 40mL NB liquid medium (each 1000mL contains 3g of beef extract, 10g of peptone, 5g of NaCl, 1000ml of water, pH7.0-7.2 ) in a 100mL Erlenmeyer flask, then placed on a shaker, and cultured with shaking at 28°C and 200rpm for 1d to obtain the seed liquid of Bacillus subtilis ZSH-1 strain, wherein the OD of the seed liquid of Bacillus subtilis ZSH-1 strain diluted 10 times 600 The value is about 0.3;

[0075] 1B) according to the inoculum size of 1% (V / V), the Bacillus subtilis ZSH-1 bacterial strain seed solution is inserted in the 100mL Erlenmeyer flask that is equipped with 40mL different salt (NaCl) concentrations, the NB liquid culture medium of pH value, in After 2 days of shaking culture at 28°C and 200rpm, pipette 0.4mL of Bacillus subtilis ZSH-1 strain seed liquid into 100mL Erlenmeyer flasks...

Embodiment 3

[0080] Example 3 Plate antagonism of Bacillus subtilis ZSH-1 bacterium liquid and sterile filtrate on poplar anthracnose

[0081] 1A) Pick a circle of activated Bacillus subtilis ZSH-1 strains and inoculate them in 100 mL of LB liquid medium (each 1000 mL contains 10 g of tryptone, 5 g of yeast extract, 10 g of NaCl, 1000 ml of water, pH 7.0 -7.2) in the 250mL Erlenmeyer flask, then the Erlenmeyer flask was placed on a shaker, and at 28°C, under the condition of 200rpm, vibration culture was carried out for 1d to prepare the Bacillus subtilis ZSH-1 bacterial strain seed liquid (i.e. ZSH-1 bacterial liquid), Among them, the OD of Bacillus subtilis ZSH-1 strain seed solution diluted 10 times 600 value is about 0.5, the seed solution is the bacterial solution used in the determination of plate antagonism, for subsequent use;

[0082] 1B) According to the inoculation amount of 1% (V / V), inoculate the ZSH-1 strain seed solution into 100mL fermentation medium (liquid) (every 1000mL...

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Abstract

The invention discloses a plant pathogenic fungus antagonistic bacterium strain and application thereof in preventing and treating plant diseases. The antagonistic bacterium is Bacillus subtilis ZSH-1, and the collection number is CGMCC NO.9025. The Bacillus subtilis ZSH-1 is separated from poplar root soil, and can prevent and treat plant diseases caused by poplar anthracnose bacterium, poplar rot bacterium, willow rot bacterium, apple rot bacterium, birchleaf pear rot bacterium, Fusarium oxysporum and date corky core bacterium. The antibacterial volatile matters generated by the antagonistic bacterium can also prevent and treat plant diseases caused by poplar anthracnose bacterium, poplar rot bacterium, willow rot bacterium, apple rot bacterium, birchleaf pear rot bacterium, Fusarium oxysporum and date corky core bacterium. The Bacillus subtilis ZSH-1 is a biological control potential strain with high prevention effect, wide preventing and treating range and high environmental safety, and has favorable development and application prospects.

Description

technical field [0001] The invention relates to a bacterial strain, in particular to an antagonistic bacterium of a plant pathogenic fungus producing bacteriostatic volatiles and application thereof, which belongs to the field of microorganisms and can be used for plant protection and plant disease prevention and treatment. Background technique [0002] The biological control of plant diseases refers to reducing the number of pathogenic bacteria or weakening the pathogenic activity of pathogenic bacteria by one or more organisms other than humans, thereby reducing the occurrence of diseases and benefiting beneficial organisms such as crops, trees, animals, beneficial insects and microorganisms . The types of microorganisms available for biological control of plant diseases include fungi, actinomycetes, bacteria, viruses and even higher plants. Among them, Bacillus subtilis is one of the most commonly used antagonistic microorganisms. [0003] Bacillus subtilis is a Gram-po...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P3/00C12R1/125
Inventor 田呈明黄华毅梁英梅于地美孙妍
Owner 北京百瑞盛田环保科技发展有限公司
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