Preparation method of thermal reaction pork essence with obvious characteristic aroma
A characteristic aroma and pork-responsive technology, which is applied in the field of food and food additives, can solve the problems of waste of resources, pollution of the environment, monotony, etc., and achieve the effect of long-lasting fragrance, full meat flavor and obvious aroma
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[0032] A method for preparing a heat-reactive pork flavor with distinct characteristic aroma, comprising the steps of:
[0033] (1) Preparation of enzymolysis-oxidized lard: according to the following method to prepare enzymolysis-oxidation fat, its peroxidation value is 50-250meq / kg, acid value is 35-55mgKOH / g fat.
[0034] The preparation of enzymolysis-oxidized lard is specifically as follows:
[0035] Add 10-50g of phosphate buffer solution with a concentration of 0.05-0.2mol / L and a pH value of 6-10 to 50-90g of pig fat to make the substrate concentration 50%-90%, according to 1000-2500U / g substrate Add lipase and enzymolyze at 30°C-50°C for 2-6 hours to obtain lipolysis product; the lipolysis product is heated and oxidized with air under mechanical stirring, the oxidation temperature is 50°C-100°C, the oxidation time is 0.5h-4h, the air flow rate is 5-150L / 100g h, the stirring speed is 100-500r / min, and the enzymolysis-oxidized lard is obtained, the peroxide value is 50...
Embodiment 1
[0047] Add 33g of 0.05mol / L pH=6.5 phosphate buffer solution (substrate concentration 60%) to 50g of refined lard, place the mixture in an enzymolysis reactor, stir slowly until the lard is melted, add 1000U / g bottom The lipase MER was hydrolyzed at 38°C for 3 hours, and the enzyme was inactivated to obtain the hydrolyzed product. Add the above pig fat enzymatic hydrolyzate into a 250mL four-necked flask equipped with an electric stirrer, a mercury thermometer, an air inlet tube and a reflux condenser, and stir the reaction with air at 80°C, the stirring speed is 150r / min, and the air flow rate is 75L / h, stop the reaction after 3h of oxidation, cool and stand for stratification, the upper layer of oxidized lard is the product, its peroxidation value is 120.36meq / kg fat, and its acid value is 46.29mgKOH / g fat.
[0048] 500g of fresh pork leg bones, cleaned and cooked under high pressure at 0.1MPa for 4h, then put into an oven for 6h at 55°C, pulverized into bone powder with a ...
Embodiment 2
[0054] Enzymolysis-oxidized lard was prepared according to the method of Example 1, the peroxidation value was 225.5meq / kg, the acid value was 45mgKOH / g fat, and it was frozen and stored for future use.
[0055] 1000g of fresh pork leg bones, cleaned and cooked under high pressure at 0.1MPa for 5h, then put into an oven for 6h at 55°C, pulverized into bone powder with a pulverizer, and set aside. A sample was taken to determine its fat content to be 22%.
[0056] Weigh 60 g of bone powder, add 140 mL of deionized water (substrate concentration is 30%), place the mixture in an enzymolysis reactor, stir evenly with magnetic force, heat up to 90 ° C for 30 min, and reduce the reaction solution to 40 ℃, adjust the pH to 7, add Lipase AY with 0.2% (0.026g) fat weight in the bone meal, and perform enzymatic hydrolysis for 2 hours; then adjust the reaction temperature to 55°C and pH 7.5, add 0.6% (0.281g) bone meal with the fat content removed After enzymatic hydrolysis for 3 hours,...
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