Method for producing D-arabitol from Hansenula polymorpha mutant strain and Hansenula polymorpha

A technology of Hansenula polymorpha and arabitol, which is applied in the field of biotransformation production of D-arabitol, can solve the problems of inability to use glucose to produce glycerol, low conversion rate of substrate glucose, insufficient phosphorus mass concentration, etc. Efficient production of D-arabitol, solving the shortage of sources, and the effect of low cost

Active Publication Date: 2014-12-10
SHAANXI UNIV OF SCI & TECH
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Problems solved by technology

However, most of the D-arabitol-producing yeast strains have a low conversion rate to the substrate glucose, and the conversion rate of most yeast strains to convert glucose to produce D-arabitol substrate glucose is between 10% and 40%. between, and the ubiquitous presence of glycerol and other polyol by-produc...

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  • Method for producing D-arabitol from Hansenula polymorpha mutant strain and Hansenula polymorpha
  • Method for producing D-arabitol from Hansenula polymorpha mutant strain and Hansenula polymorpha
  • Method for producing D-arabitol from Hansenula polymorpha mutant strain and Hansenula polymorpha

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[0031] The present invention will be described in detail below in conjunction with the accompanying drawings and embodiments. The examples are used to illustrate the present invention, but not to limit the scope of the present invention.

[0032] One of the objectives of the present invention is to provide a Hansenula polymorpha acid phosphatase gene (PHO1) mutant strain.

[0033] The second object of the present invention is to provide a method for producing D-arabitol by biotransformation using the mutant strain.

[0034] The third object of the present invention is to provide the application of the mutant strain in biotransformation production of D-arabitol.

[0035] Using genetic engineering methods, construct the gene knockout plasmid pMD18-PHO-kanMX, use PCR method to amplify the PHO1 gene knockout fragment, combine conventional electroporation method to transform Hansenula polymorpha, use high phosphorus medium chromogenic screening method and PCR Methods The mutant s...

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Abstract

The invention provides a method for producing D-arabitol from Hansenula polymorpha mutant strain and Hansenula polymorpha. A gene engineering technique is mainly utilized to obtain a D-arabitol high-yield mutant strain by establishing a Hansenula polymorpha acid phosphatase gene mutant strain. The method has the advantages of low cost and short production cycle, is easy to operate, and provides a new way for solving the problem of shortage of D-arabitol sources. The mutant strain is supplemented by batches through glucose to perform biotransformation so as to produce the D-arabitol; and the conversion rate of the substrate glucose is 51.2%, which is obviously higher than the conversion rate of the substrate glucose when the initial strain is supplemented by batches through glucose to perform biotransformation so as to produce the D-arabitol.

Description

technical field [0001] The invention belongs to the technical field of biological fermentation, and specifically relates to a mutant strain constructed by knocking out the acid phosphatase gene of Hansenula polymorpha by using a gene knockout method, and using the mutant strain to produce D-arabinitol by a biotransformation method. Background technique [0002] D-arabitol is a five-carbon sugar alcohol with the molecular formula C 5 h 12 0 5 , with a molecular weight of 152.12, is an isomer of xylitol and ribitol. It is a sweet white crystal with a melting point of 103°C and an optical rotation of 130°. It is highly hygroscopic in air. Sugar alcohols are a type of polyols reduced by sugar. Due to their low calorific value, anti-caries, and no effect on insulin levels, they have important application values ​​in the fields of medicine, food, and chemical industry. [0003] In the food field, D-arabitol can be used as an additive to improve the quality of alcoholic beverag...

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Application Information

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IPC IPC(8): C12N1/16C12P7/18C12R1/78
Inventor 钱卫东王婷毛培宏宁肖肖
Owner SHAANXI UNIV OF SCI & TECH
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