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Positive control applied to quantitative detection of protein chip and preparation method

A quantitative detection and protein chip technology, which is applied in the field of positive reference, can solve the problems of washing off the lotion, affecting the quantitative accuracy of the target, and weak binding, etc., and achieve the effect of reducing errors

Active Publication Date: 2014-12-10
北京九州泰康生物科技有限责任公司 +2
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to the weak combination, it is easy to be washed off by the washing solution, which affects the accuracy of quantification of the target.
Moreover, this type of reference point can only characterize the reaction from biotin and horseradish peroxidase (HRP) to the color reaction in the hybridization reaction, but cannot characterize the antigen-antibody reaction, which also affects the accuracy of the quantification of the target.

Method used

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  • Positive control applied to quantitative detection of protein chip and preparation method
  • Positive control applied to quantitative detection of protein chip and preparation method

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Embodiment Construction

[0022] The positive reference applied to the quantitative detection of protein chips provided by the present invention utilizes the characteristic that antibody molecules can specifically bind to antigen molecules, mixes a kind of artificially synthesized polypeptide into the sample (especially serum), and combines the polypeptide with the antibody substance The luminescence value of the target object is used as a reference, and the measured luminescence value of the target object is compared with it to obtain a relative value, which is used for quantitative analysis of the target object in the sample.

[0023] The positive reference of the present invention comprises the following parts:

[0024] 1) FLAG coated antibody;

[0025] 2) HA-FLAG polypeptide, its basic sequence is: YPYDVPDYA-C-C-C-C-C-C-C-DYKDDDDK

[0026] 3) HA biotin-labeled antibody

[0027] A "sandwich" compound composed of the above three.

[0028] The present invention gives examples to further illustrate ...

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Abstract

The invention discloses a positive control applied to quantitative detection of a protein chip. 1) a FLAG coated antibody, 2) HA-FLAG polypeptide and 3) an HA biotin labeled antibody are located on a biochip in sequence. The invention further discloses a method for preparing the positive control. The control can characterize whether a whole experiment from antigen and antibody response to development is successful, serves as a reference substance to quantitatively analyze targets in blood serum, and overcomes the defects of poor reliability and failure in standardization in the prior methods.

Description

technical field [0001] The invention relates to a positive reference applied to quantitative detection of protein chips. [0002] The present invention also relates to a method for preparing the above-mentioned positive reference substance. Background technique [0003] The technical principle of the protein chip was first proposed by Roger Ekin in the 1980s. It fixes a large number of protein molecules on the surface of a carrier in a preset arrangement to form a microarray. According to the principle of specific binding between protein molecules, a microarray is constructed. Fluid biochemical analysis system to achieve accurate, rapid and large-information detection of biomolecules. Protein chip technology is mainly used for chip-based proteomics research, which is characterized by high throughput, that is, thousands of proteins are labeled on a chip substrate of only tens of square millimeters for the detection of other target proteins. The basic principle is to use the...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68
CPCG01N33/532G01N33/68
Inventor 丁立功马君兰臧百盛宫晓艳
Owner 北京九州泰康生物科技有限责任公司