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Willow liquid hybridization pollination method

A technology of hybrid pollination and willow, which is applied in the direction of botany equipment and methods, application, plant genetic improvement, etc., can solve the problems of poor suspension capacity of nutrient solution, uneven dispersion of pollen, easy swelling and necrosis of pollen, etc., so as to save costs and improve The effect of pollination efficiency and labor cost saving

Active Publication Date: 2014-12-17
INST OF AGRI SCI ALONG YANGTZE RIVER IN JIANGSU
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  • Summary
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  • Application Information

AI Technical Summary

Problems solved by technology

However, the liquid pollination technology is still immature, and there are problems such as poor suspending ability of the nutrient solution, uneven dispersion of pollen, and easy swelling and necrosis of pollen in the nutrient solution.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0015] A willow liquid cross pollination method, the steps are as follows:

[0016] (1) Collection of pollen

[0017] Select willow male flowers in the big bud stage in the field and collect them at 8:30 in the morning. After harvesting, spread them on sulfuric acid paper, and place them naturally for a day and night under dry and ventilated conditions at 25°C. After the pollen is completely burst from the anthers, use them Sieve through an 80-mesh sieve, remove the anther residues, place the pollen in a petri dish, put it in a desiccator, and put it in a refrigerator at 0-5°C for later use;

[0018] (2) Preparation of willow pollen liquid nutrient solution

[0019] Dissolve starch sodium octenyl succinate as a pollen dispersant in 60°C hot water and cool to room temperature, then add willow pollen liquid culture medium, stir to mix well, then add pollen, the pollen concentration is 0.6g / L, stirring, the pollen is evenly dispersed in the solution, and the special pollen liq...

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Abstract

The invention discloses a willow liquid hybridization pollination method, including the steps of pollen collection, preparation of a special pollen liquid nutrient solution for willow and pollination. The invention reaches the goals of saving cost and improving pollination efficiency, and can save labor cost by 65% and increase pollination efficiency by 37%.

Description

technical field [0001] The invention relates to a willow liquid cross pollination method. Background technique [0002] The existing willow hybrid pollination method mainly adopts the method of manual point pollination. The traditional method takes a long time to operate, requires a lot of labor, and the production cost is relatively high. The liquid pollination technology has fast pollination speed, saves labor, and can quickly replenish water and nutrients to the stigma, thereby prolonging the pollination time of the stigma. However, the liquid pollination technology is still immature, and there are problems such as poor suspending ability of the nutrient solution, uneven dispersion of pollen, and easy swelling and necrosis of pollen in the nutrient solution. Contents of the invention [0003] The purpose of the present invention is to provide a willow liquid hybrid pollination method which saves cost and improves pollination efficiency. [0004] Technical solution of ...

Claims

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Application Information

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IPC IPC(8): A01H1/02
Inventor 李敏张健马祥建李玉娟王莹谈峰
Owner INST OF AGRI SCI ALONG YANGTZE RIVER IN JIANGSU
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