Willow liquid hybridization pollination method
A technology of hybrid pollination and willow, which is applied in the direction of botany equipment and methods, application, plant genetic improvement, etc., can solve the problems of poor suspension capacity of nutrient solution, uneven dispersion of pollen, easy swelling and necrosis of pollen, etc., so as to save costs and improve The effect of pollination efficiency and labor cost saving
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[0015] A method for liquid hybrid pollination of willows, the steps are as follows:
[0016] (1) Collection of pollen
[0017] Select willow male flowers in the large bud stage in the field and collect them at 8:30 in the morning. After harvesting, spread them on sulfuric acid paper and place them naturally at 25°C under dry and ventilated conditions for a day and night, and use them after the pollen has completely exploded from the anthers. Sieve through an 80-mesh sieve to remove the anther residue, place the pollen in a petri dish, put it in a desiccator, and place it in a refrigerator at 0~5℃ for later use;
[0018] (2) Preparation of willow pollen liquid nutrient solution
[0019] First dissolve the starch sodium octenyl succinate as a pollen dispersant with hot water at 60℃ and cool to room temperature, then add the willow special pollen liquid culture solution, stir to fully mix, then add the pollen, the concentration of the pollen is 0.6g / L, stir to disperse the pollen evenl...
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