Adonis amurensis extract or anti-cancer composition using same as an active ingredient

A kind of technology of Aphrodisiac sativa and its composition, which is applied in the field of anticancer agent composition

Active Publication Date: 2014-12-17
济州岛科技园
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Currently used anticancer agents can be roughly divided into biological agents such as enzyme preparations or vaccines, chemically synthesized drugs, and drugs from natural organisms. Among them, biological agents such as enzymes and vaccines have not yet been applied, while chemically synt...

Method used

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  • Adonis amurensis extract or anti-cancer composition using same as an active ingredient
  • Adonis amurensis extract or anti-cancer composition using same as an active ingredient
  • Adonis amurensis extract or anti-cancer composition using same as an active ingredient

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] Manufacture of extracts and fractions of A.

[0061] Put 1 kg of finely chopped Azalea (whole herb) into 80% ethanol, soak for 48 hours, extract repeatedly three times, filter to obtain the extract, concentrate the extract under reduced pressure to remove the solvent, and freeze-dry to obtain a powder extract.

[0062] In addition, after suspending and dissolving the obtained ethanol extract in 10 wt. 2 Cl 2 ), ethyl acetate (EtOAc) butanol (BuOH) for fractional distillation to obtain fractions of a hexane layer, a dichloromethane layer, an ethyl acetate layer, a butanol layer and the remaining aqueous layer.

Embodiment

[0064] Hereinafter, the present invention will be described with reference to examples and experimental examples. However, the scope of the present invention is not limited by these Examples and Experimental Examples.

[0065] Manufacture of the extract and fraction of A. sativa and the separation of its effective substances

[0066] Manufacture of extracts and fractions of A.

[0067] Put 1 kg of finely chopped Azalea (whole herb) into 80% ethanol, soak for 48 hours, extract repeatedly three times, filter to obtain the extract, concentrate the extract under reduced pressure to remove the solvent, and freeze-dry to obtain a powder extract.

[0068] In addition, after suspending and dissolving the obtained ethanol extract in 10 wt. 2 Cl 2 ), ethyl acetate (EtOAc) butanol (BuOH) for fractional distillation to obtain fractions of a hexane layer, a dichloromethane layer, an ethyl acetate layer, a butanol layer and the remaining aqueous layer.

Embodiment 2

[0069] Separation and identification of effective substances

[0070] 8 g of the above-mentioned ethyl acetate fraction was mixed with Celite 545, and the resulting mixture was concentrated under reduced pressure, followed by open column chromatography. Using n-Hex., MC (methylene chloride, dichloromethane), DE (Diethyl ether, diethyl ether), EA (Ethyl acetate, ethyl acetate), Methaneol (MeOH, methanol) as the mobile phase to obtain a total of five small fractions . However, silica gel column chromatography was performed on the DE (800 mg) fraction. Using chloroformate / methanol (9 / 2) as mobile phase at a flow rate of 2.5 ml / min, a total of 18 small fractions were obtained. The No. 14 fraction (DE-14) was simply purified to obtain a single compound (compound1).

[0071] To analyze the structure of the isolated substance, 10 mg of a single compound was dissolved in methanol-d to measure NMR spectrum. In 1H NMR spectrum ( figure 1 and image 3 ) in which three methyl group...

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Abstract

The present invention relates to an Adonis amurensis extract showing anti-cancer activity in cell and animal experiments, and an anti-cancer composition using the same as an active material.

Description

technical field [0001] The present invention relates to an anticancer drug composition using an extract of Adonis amurensis or its active substance. Background technique [0002] In general, cancer refers to a malignant tumor in which the cycle of cells constituting human tissues is abnormal, resulting in abnormal differentiation of cells, regulation of growth, and enlargement of cells. [0003] According to the known situation, cancer occurs through three steps of initiation, promotion, and progression. Cell mutations are caused by carcinogens contained in the environment or food. Such cells survive the persistence of carcinogens. Under the stimulation, abnormal proliferation continues to form cancerous tissue. [0004] The research methods of anticancer agents for the treatment of cancer include the method of searching for direct cytotoxic substances to cancer cells, the method of searching for substances that can regulate the immune ability of the human body, the method ...

Claims

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Application Information

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IPC IPC(8): A61K31/35A61P35/00A61K36/71A61K9/20
CPCA61K31/352A61K36/71A23L33/105A61P35/00A61K2300/00A61K9/20A61K31/35
Inventor 咸瑛旻金佶男郑龙焕尹源种朴修永吴大株
Owner 济州岛科技园
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