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Method for imparting environmental stress tolerance to plants

An environmental and tolerance technology, applied in the field of environmental stress tolerance, can solve problems such as incompatibility, and achieve the effects of increasing production, realizing cost reduction, and cost reduction.

Inactive Publication Date: 2017-03-01
TOYOTA JIDOSHA KK
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

An environment sometimes characterized by these elements is suitable for some plants but not for others

Method used

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  • Method for imparting environmental stress tolerance to plants
  • Method for imparting environmental stress tolerance to plants
  • Method for imparting environmental stress tolerance to plants

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0096] (1) Selection of Arabidopsis tyrosine phosphatase (PTP) gene

[0097] In this example, a gene specified by the AGI code: At1g71860 was selected as the tyrosine phosphatase gene in Arabidopsis. In addition, this At1g71860 is known in Plant Cell, 10, 849-857, 1998 as a gene characterized by enhanced expression by high-salt concentration treatment. In this example, the possibility of growth and development under high-salt concentration conditions was investigated for a mutant in which the At1g71860 gene was suppressed.

[0098] In addition, the nucleotide sequence of the coding region of the At1g71860 gene is shown in SEQ ID NO: 1, and the amino acid sequence of the tyrosine phosphatase encoded by this gene is shown in SEQ ID NO: 2.

[0099] (2) T-DNA tag line of Arabidopsis

[0100] Arabidopsis T-DNA insertion mutants in which T-DNA was inserted into the selected At1g71860 gene, SALK_118658 and SALK_062441 obtained from Nottingham Arabidopsis Stock Center (http: / / nasc.n...

Embodiment 2

[0113] In this example, an overexpressor of the At1g71860 gene and an overexpressor of the mutant At1g71860 gene were prepared, and the possibility of growth and development under high-salt concentration conditions was investigated in the same manner as in Example 1. In addition, the mutant At1g71860 gene produced in this example encodes an amino acid sequence in which cysteine ​​at position 175 in the amino acid sequence shown in SEQ ID NO: 2 is replaced by serine, para-oxidized glutathione and / or Or the gene for hydrogen peroxide-hyposensitivity tyrosine phosphatase.

[0114] (1) Overexpression body of At1g71860 gene

[0115] A nucleic acid fragment comprising the At1g71860 gene was cloned from Col by the following steps.

[0116] First, total RNA was recovered from Col using RNeasy Plant Mini Kit (manufactured by Qiagen). Genomic DNA was digested with DNase I (manufactured by Invitrogen), and then cDNA was prepared from 5 μg of total RNA using StrataScript First Strand cD...

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Abstract

Imparting environmental stress tolerance to plants or improving environmental stress tolerance of plants. Environmental stress tolerance is imparted to plants by changing the expression level of a tyrosine phosphatase gene (At1g71860).

Description

technical field [0001] The present invention relates to a method for imparting environmental stress tolerance to plants by changing the expression level of a predetermined gene. Background technique [0002] The growth and development potential of plants depends on various environmental factors such as temperature, humidity, and salt concentration contained in the soil. Sometimes the environment characterized by these elements is suitable for some plants but not for others. Generally, the above-mentioned factors that affect the growth and development of plants are called environmental stress. In an environment with specified environmental stress, if the specified plant cannot grow or develops with difficulty, it means that the plant has no environmental stress tolerance. In contrast, a plant that grows and develops even in an environment with a defined environmental stress indicates environmental stress tolerance. [0003] If environmental stress tolerance can be imparted...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/00A01H1/00C12N15/09
CPCC12N15/8271C12N9/16C12Y301/03048C12N15/8273
Inventor 近藤聪大音德小川健一逸见健司
Owner TOYOTA JIDOSHA KK
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