Cerebroprotein hydrolysate and preparation method thereof

A cerebroprotein hydrolysate and pepsin technology, applied in the field of medicine, can solve the problems of different types, compositions and ratios, different effects, different production processes, etc., and achieve high anti-oxidative stress activity, good repair ability, and improved effect. Effect

Active Publication Date: 2014-12-24
BEIHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main reason may be that the production process of each manufacturer is different, and the types, compositions and ratios of amino acids and small molecule peptides in the obtained products are different, resulting in different effects

Method used

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  • Cerebroprotein hydrolysate and preparation method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] A cerebroprotein hydrolyzate prepared by the following method:

[0028] (1) Enzymolysis:

[0029] Take healthy porcine brain tissue, add physiological saline at an amount of 2 mL per gram of pig brain, homogenize with a low-temperature ultramicro cell wall breaker, adjust the pH value to 3.0 with phosphoric acid, freeze and thaw repeatedly 3 times, and adjust the total enzyme amount to 0.65 mg enzyme / g brain tissue, add pepsin and compound flavor protease, the mass ratio of the added pepsin and compound flavor protease is 4:1, and enzymolyze at 37°C for 4 hours to obtain an enzymatic hydrolysis solution.

[0030] (2) Impurity removal:

[0031] Adjust the pH value of the enzymolysis solution with calcium hydroxide to 7, centrifuge at 6,000 rpm to obtain a clarified supernatant, and filter the supernatant with a GE-TFM-MWCO1000 nanofiltration membrane from GE Company of the United States to obtain cerebroprotein-containing Filtrate of hydrolyzate.

[0032] (3) drying: ...

Embodiment 2

[0035] A cerebroprotein hydrolyzate prepared by the following method:

[0036] (1) Enzymolysis:

[0037] Take healthy bovine brain tissue, add physiological saline at an amount of 1 mL per gram of bovine brain, homogenize with a low-temperature ultramicro cell wall breaker, adjust the pH value to 1.0 with phosphoric acid, freeze and thaw twice, and adjust the total enzyme amount to 1.0 mg enzyme / g brain tissue, add pepsin and compound flavor protease, the mass ratio of the added pepsin and compound flavor protease is 6:1, and enzymolyze at 37°C for 2 hours to obtain an enzymatic hydrolysis solution.

[0038] (2) Impurity removal:

[0039] Use calcium hydroxide to adjust the pH value of the enzymolysis solution to 6, centrifuge at 5000 rpm to obtain a clarified supernatant, and filter the supernatant with a GE-TFM-MWCO1000 nanofiltration membrane from GE Company of the United States to obtain cerebroprotein-containing Filtrate of hydrolyzate.

[0040] (3) drying:

[0041] T...

Embodiment 3

[0043] A cerebroprotein hydrolyzate prepared by the following method:

[0044] (1) Enzymolysis:

[0045] Take healthy pig brain tissue, add 3 mL of normal saline per gram of pig brain, homogenate with a low-temperature ultramicro cell wall breaker, adjust the pH value to 5.0, freeze and thaw repeatedly 4 times, and use a total enzyme amount of 0.3 mg enzyme Add pepsin and compound flavor protease per gram of brain tissue, the mass ratio of the added pepsin and compound flavor protease is 2:1, and enzymolyze at 37°C for 10 hours to obtain an enzymatic hydrolysis solution.

[0046] (2) Impurity removal:

[0047]Adjust the pH value of the enzymolysis solution to 7.5, centrifuge at 7000 rpm to obtain a clarified supernatant, and filter the supernatant with a GE-TFM-MWCO1000 nanofiltration membrane from GE Company of the United States to obtain a filtrate containing cerebroprotein hydrolyzate .

[0048] (3) drying:

[0049] The filtrate containing cerebroprotein hydrolyzate obt...

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Abstract

The invention discloses a cerebroprotein hydrolysate and a preparation method thereof, and belongs to the technical field of medicines. The preparation method comprises the following step: carrying out enzymolysis on an animal brain tissue by adopting pepsase and compound flavourzyme in a mass ratio of (6:1)-(2:1) at 37 DEG C under a corresponding enzymolysis pH value controlled at 1-5 for 2-10 hours, thereby obtaining the cerebroprotein hydrolysate, wherein cerebroprotein hydrolysate is a composition of oligopeptide with molecular weight of 1000 and polypeptide. And moreover, macromolecule impurities are removed by centrifuging, and impurities such as pyrogen are filtered and removed by a nanofiltration membrane. The finally obtained cerebroprotein hydrolysate has apyrogeneity, high oxidative-stress-resisting activity and good repair capacity on nerve cells.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to a cerebroprotein hydrolyzate and a preparation method thereof. Background technique [0002] With the natural population growth, aging structure, and changes in diet structure, the occurrence of senile diseases is becoming more and more serious. Among them, cerebrovascular disease is one of the three major causes of death among senile diseases. Cerebroprotein hydrolyzate drugs have attracted widespread attention in recent years because of their special curative effect on brain dysfunction diseases. Cerebroprotein hydrolyzate drugs that have been marketed can be divided into tablets, oral liquids and injections. The main ingredients are a mixture of free amino acids and small molecule peptides with a molecular weight below 10,000 produced by protease degradation after degreasing animal brains. [0003] In recent years, the variety of such drugs produced at home and abroad has b...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/01A61K35/30A61P9/10A61P25/00
Inventor 杜培革王曼力安丽萍李洪宇苑广信徐广宇韩笑盛瑜王广红陈丽娜张濛川叶玉洁侯志东仪忠勋
Owner BEIHUA UNIV
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