Device and method for rapidly and immediately separating epidermal cells, melanophore and fibroblast of human skin

A technology for melanocytes and fibroblasts, applied in the field of medical cell therapy, can solve the problems of many steps of separating cells, complicated operations, difficult to popularize and apply, etc., and achieves the effects of stable quantity and activity, simple operation process, and convenient clinical popularization.

Inactive Publication Date: 2014-12-24
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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Problems solved by technology

The above-mentioned method has many steps, takes a long time, and requires special instruments and equipment. It is difficult to perform synchronously with the operation in the operating room, which has become the main obstacle to the popularization and application of human skin cell therapy. Or cold digestion, the whole cell separation process takes 2-16 hours, and the separated cells are difficult to be applied in the operating room in a short time; 2. There are many steps to separate the cells: the first digestion is required to separate the epidermis, and the second digestion is required to separate the epidermis Cell components, then filter, centrifuge, resuspend cells, etc. The operation is relatively complicated, requires certain equipment and related technologies, and can only be carried out in laboratories with certa

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  • Device and method for rapidly and immediately separating epidermal cells, melanophore and fibroblast of human skin

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Embodiment 1

[0054] Such as figure 1 As shown, a device for rapidly and instantly separating human skin epidermal cells, melanocytes and fibroblasts, the device includes: a magnetic heating stirrer 1 and an operation panel 2, and the operation panel 2 is embedded on the magnetic heating stirrer 1 , the two form a whole;

[0055] The operation panel is provided with 5 holes, one digestion hole 3A; three rinsing holes 3B, 3C, 3D; one magnetic stirring and filtering hole 3E, the magnetic stirring and filtering hole is equipped with a removable 80 mesh -200 mesh filter 4. In the magnetic stirring and filtering hole, a magnetic stirring bar is placed in it.

[0056] The magnetic heating stirrer also includes a temperature indicator 5 and a rotation speed indicator 6, the temperature can be controlled between 25-40 degrees Celsius, and the stirring speed is 100-1200rpm.

[0057] The 3A hole is circular, with a diameter of 3cm and a depth of 3cm. The temperature is precisely controlled at 25-4...

Embodiment 2

[0066] Utilize the device of embodiment 1, the method for separating human skin epidermal cells, melanocytes and fibroblasts comprises the following steps:

[0067] 1. Dissolve 0.001-0.02 grams of digestive enzymes (collagenase, neutral protease and trypsin at a ratio of 1:1:1) in 2-10ml isotonic electrolyte solution D-hank's solution, and prepare a mass concentration of 0.1%- Add 1% digestive solution into well A, start the micro-magnetic heating stirrer, preheat for 5-10 minutes, and make the solution temperature 25-40 degrees Celsius;

[0068] 2. Cut the skin slices from any part of the human body, with a thickness of about 0.15-0.6mm and a size of 0.5cm 2 -16cm 2 Wait;

[0069] 3. Place the cut slices in well 3A and incubate for 5-45 minutes;

[0070] 4. Add electrolyte solution such as Hank’S solution to wells 3B, 3C, and 3D, take out the incubated tomographic skin, and rinse 1-3 times in wells 3B, 3C, and 3D respectively;

[0071] 5. Place the rinsed fractured skin f...

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Abstract

The invention relates to the field of medical cell therapy, in particular to a device and a method for rapidly and immediately separating epidermal cells, melanophore and fibroblast of human skin. The separating device comprises a micro magnetic heating stirrer, an operating panel, digestive enzyme and an isotonic electrolyte solution. According to the separating method, instruments required for cell separation of the human skin are remarkably reduced, the operation steps are simplified, time required for cell separation is remarkably shortened, application of serum is avoided, occurrence of serious complication such as allergy, pathogenic microorganism propagation and the like is remarkably reduced, and wide popularization of human skin cell transplantation application is facilitated. According to the device and the method, separated cells can be used for scar plastic surgery, treatment of abnormal pigment change such as vitiligo and scar hypopigmentation caused by various reasons and acute and chronic wound repair.

Description

technical field [0001] The present invention relates to the field of medical cell therapy, in particular to a device for rapidly and instantly separating epidermal cells, melanocytes and fibroblasts from human skin, as well as a separation method; the cells separated by the present invention can be used for scar shaping and various reasons Treatment of abnormal pigment changes (such as vitiligo, cicatricial hypopigmentation) and repair of acute and chronic wounds. Background technique [0002] With the development of medical technology, cell therapy has become one of the important treatment methods for many diseases, and has attracted extensive attention from researchers and clinicians. The main cell components in human skin are epidermal cells, fibroblasts and melanocytes. The above cells are isolated and applied immediately (that is, without in vitro culture and expansion), or delayed application (that is, in vitro culture and expansion), combined with other treatments Th...

Claims

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Application Information

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IPC IPC(8): C12M1/12C12M1/02C12M1/00C12N5/071
CPCC12M47/04C12N5/0625C12N5/0626C12N5/0656C12N2509/00
Inventor 肖仕初田松夏照帆吴海斌郑勇军王志红纪世召
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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