Method for culturing microalgae and method for producing grease

A technology of microalgae and algae liquid, applied in the field of microalgae cultivation, can solve the problems of unfavorable clear liquid, increased cost, and influence on the vitality of microalgae, and achieve the effect of simple operation and normal growth

Active Publication Date: 2014-12-24
CHINA PETROLEUM & CHEM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This method still has the following deficiencies: ① adjusting the strong alkalinity of pH to make it self-flocculate will lead to an increase in cost and affect the viability of microalgae, which is not conducive to the recycling of algae species; ② in some cases, slight disturbance will make microalgae Algae redispersion, which is not conducive to the separation of the settled supernatant

Method used

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  • Method for culturing microalgae and method for producing grease
  • Method for culturing microalgae and method for producing grease
  • Method for culturing microalgae and method for producing grease

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] The cultivation of Chlorella is carried out by adopting the two-stage expanded basic technological process. Primary inoculation culture: 1L glass triangular flask filled with about 700ml medium, the medium is BG11 medium (Table 1), primary inoculation medium uses urea as nitrogen fertilizer, the N content of the algae liquid after inoculation is 40mmol / L, control the light And the temperature is suitable, and 2% (v / v) CO is introduced 2 / Air mixed gas is used for aeration culture, and the mixed gas is filtered and purified by filter membrane. Detect the OD value of the algae liquid. When the OD value of the algae liquid reaches more than 5, the secondary expansion culture can be carried out. Secondary expansion culture: The expansion culture is carried out in a 5L glass triangular flask, filled with 3-4L medium (the mother liquid of the medium is sterilized by high pressure, high temperature or boiled, and the culture water is filtered and sterilized tap water), and th...

Embodiment 2

[0046] The culture of chlorella, method is the same as example 1, and difference is that the N content of algae liquid after inoculation is 66mmolg / L. Growth curve see figure 1 .

Embodiment 3

[0048] The culture of chlorella, method is the same as example 1, and difference is that the N content of algae liquid after inoculation is 100mmol / L.

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Abstract

The invention relates to a method for culturing microalgae and a method for producing grease. The method for culturing the microalgae comprises a step of harvesting the microalgae after over 10 days of photoautotrophic cultivation in an inoculated algae fluid, with a concentration of nitrogen fertilizer higher than 30 mmol/L, in terms of nitrogen atoms. The method provided by the invention can be used for culturing the microalgae efficiently at a large scale and low cost, can guarantee rapid growth of the microalgae, and enables the microalgae to have relatively high grease content.

Description

technical field [0001] The invention relates to a method for cultivating microalgae and a method for producing oil. Background technique [0002] Microalgae are a class of low-level plants that grow in water with a wide variety and are extremely widely distributed. It is a cell factory driven by sunlight, and absorbs CO through efficient photosynthesis of microalgae cells. 2 , convert light energy into chemical energy of carbohydrates such as fat or starch, and release O 2 . The use of microalgae to produce bioenergy and chemicals can simultaneously achieve "replacing fossil energy and reducing CO 2 The three purposes of "emission, purification of waste gas and sewage". The advantages of "microalgae biotechnology" lie in the following aspects: ① Microalgae is the original plant with the highest photosynthetic efficiency. Compared with crops, the yield per unit area is dozens of times higher Microalgae is also the fastest-growing plant in nature, usually within 24 hours, t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/38C12P7/64C12R1/89
Inventor 荣峻峰周旭华朱俊英黄绪耕程琳
Owner CHINA PETROLEUM & CHEM CORP
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