Human seminal fluid cryoprotectant with Chinese medicine monomer

A cryoprotectant and human semen technology, applied in the preservation, application, and animal husbandry of human or animal bodies, can solve the problems of lack of systematic research on new cryoprotectants, complex components of traditional Chinese medicine extracts, and difficulty in receiving international recognition. , to achieve excellent semen preservation effect and improve the preservation effect

Inactive Publication Date: 2015-01-07
JIANGSU PROVINCIAL HOSPITAL OF TCM
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Problems solved by technology

However, with the accumulation of clinical experience, we found that various semen cryoprotectants on the market are less effective in freezing the semen of patients with oligoasthenospermia
The fifth edition of the WHO "Laboratory Manual for Analysis and Processing of Human Semen" published at the end of 2009 listed a separate chapter for the first time discussing the standardized process of semen freezing, and also mentioned the particularity of cryoprotectants for oligospermia and weak spermatozoa. Cryopreservation h

Method used

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  • Human seminal fluid cryoprotectant with Chinese medicine monomer
  • Human seminal fluid cryoprotectant with Chinese medicine monomer
  • Human seminal fluid cryoprotectant with Chinese medicine monomer

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Embodiment Construction

[0019] Experimental samples and materials:

[0020]The present invention selects the semen routine examination specimen of outpatient department of reproductive medicine, 3 parts of normal semen, 10 parts of less and weak sperm.

[0021] For normal semen, choose semen with good density and motility rate, and for less weak sperm, choose semen with a density of 10-20×10 6 , Semen with a better motility rate.

[0022] Sperm quality before normal semen processing (sperm and cryoprotectant mixed in a volume ratio of 1:1 or 1:2):

[0023] Density: 45±5.0×10 6 ; Motility rate: 70.67%±5.13; a+b (percentage of forward motile sperm): 51.00%±5.20; c (percentage of non-forward motile sperm): 19.67%±3.79

[0024] Sperm quality before oligo-weak sperm treatment (sperm and cryoprotectant are mixed at a volume ratio of 1:1):

[0025] Density: 16.00±2.45×10 6 ; Motility rate: 61.90% ± 6.12; a+b (percentage of forward motile sperm): 43.20% ± 5.98; c (percentage of non-forward motile sperm)...

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Abstract

The invention provides a human seminal fluid cryoprotectant with a Chinese medicine monomer. The cryoprotectant comprises the Chinese medicine monomer and a human seminal fluid basic cryoprotectant, wherein the Chinese medicine monomer is hyperoside or icariin. By adding the traditional Chinese monomer hyperoside or icariin into the conventional basic seminal fluid cryoprotectant and screening the proportional relation of the Chinese medicine monomer and the basic seminal fluid cryoprotectant, so that the human seminal fluid cryoprotectant with an excellent performance can be prepared, and an excellent seminal fluid preservation effect can be achieved by matching a rapid freezing and rapid thawing method; meanwhile, a novel way is developed for the application of the Chinese medicine monomer in the seminal fluid cryoprotectant, not only can the situation that the preservation effect of sperms can be effectively improved by adding the Chinese medicine monomer in the seminal fluid cryoprotectant be proved, but also a complete freezing system which is likely to accept by the international market and suitable for the people with weak sperms can be established, and the difficulty for freezing the weak sperms can be solved.

Description

technical field [0001] The invention belongs to the technical field of cryoprotectants for human semen, in particular to a cryoprotectant for human semen added with traditional Chinese medicine monomers. Background technique [0002] Semen cryopreservation has a history of more than 200 years. The cryopreservation of human semen dates back to the late 1940s. At present, semen cryopreservation is mainly used in human sperm banks and preservation of fertility in my country. Researchers at home and abroad have established a relatively mature semen cryopreservation system through decades of screening of semen freezing procedures, freezing diluents, and thawing fluids. Reproductive centers across the country have a relatively large demand for cryoprotectants for human semen, but almost all of the cryopreservatives for human semen on the market are monopolized by foreign biological companies, and only some scientific research institutes in China configure cryoprotectants for liv...

Claims

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Application Information

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IPC IPC(8): A01N1/02
Inventor 聂晓伟谈勇陈娟邹奕洁
Owner JIANGSU PROVINCIAL HOSPITAL OF TCM
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