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A method for optimizing the effect of vitrification cryopreservation of Cymbidium protocorms

A vitrified ultra-low temperature, protocorm-like technology, applied in the preservation of plants, botanical equipment and methods, applications, etc., can solve problems such as insufficient production and scientific research, and achieve the improvement of relative survival rate, optimization of preservation effect, and improvement of Save the effect of the effect

Active Publication Date: 2016-04-06
SHANGHAI JIAOTONG UNIV
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  • Application Information

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Problems solved by technology

The relative survival rate of the vitrified cryopreservation system of Cymbidium protocorms that has been initially established is only 10%, which is not enough to meet the needs of production and scientific research

Method used

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  • A method for optimizing the effect of vitrification cryopreservation of Cymbidium protocorms

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] 1) Precultivating Cymbidium protocorms under light for 5 days on MS solid medium containing 60g / L sorbitol;

[0052] 2) Transfer to the loading solution and soak at room temperature for 40 minutes;

[0053] 3) Transfer to vitrification solution and dehydrate at 0°C for 50 minutes;

[0054] 4) Finally, store in liquid nitrogen at ultra-low temperature.

[0055] After step 3), without removing the vitrification solution, the Cymbidium-like protocorms soaked in the vitrification solution are directly stored in liquid nitrogen at ultra-low temperature.

[0056] Cymbidium protocorms were divided into experimental group and control group according to the above steps.

[0057] Among them, the vitrification solutions of the experimental groups contained graphene quantum dots of 0.1 g / L, 0.3 g / L, and 0.5 g / L respectively.

[0058] Specifically, the vitrification solution of experimental group 1 contained 0.1 g / L graphene quantum dots.

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Abstract

The invention discloses a method for optimizing a vitrification ultra-low temperature preservation effect of cymbidium type protocorm. According to the invention, the cymbidium type protocorm is processed by adopting a vitrification solution which contains a carbon nanometer material so as to improve the preservation effect of the cymbidium type protocorm. The method specifically comprises the steps of preculture, loading liquid treatment, vitrification solution treatment and liquid nitrogen preservation, wherein the vitrification solution contains 0.1-0.5 g / L of grapheme quantum dots. The method disclosed by the invention optimizes the preservation effect of the cymbidium type protocorm remarkably; as the grapheme quantum dots are added to serve as an allogenic material, the vitrification ultra-low temperature preservation of plants is facilitated.

Description

technical field [0001] The invention relates to the field of preservation of plants or parts thereof, in particular to a method for optimizing the ultralow temperature preservation effect of vitrification of protocorms of Cymbidium grandiflorum. Background technique [0002] Cryopreservation is a modern in vitro preservation technology for germplasm resources developed in the 1970s. Usually stored in liquid nitrogen, the substance metabolism and growth activities in the preserved material cells are almost completely stopped, and they are in a relatively stable biological state, achieving the purpose of long-term preservation of germplasm. Ultra-low temperature preservation is currently the only method that does not require continuous subculture. Medium and long-term preservation method. Vitrification cryopreservation is to place cells or tissues in a vitrification solution composed of a certain proportion of permeable and non-permeable protective agents, so that the materia...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N3/00
Inventor 张荻王路尧陈冠群任丽
Owner SHANGHAI JIAOTONG UNIV