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Rice brown planthopper resistance gene bph28 and its application

An anti-BPH gene technology, applied in the fields of molecular biology and agriculture

Active Publication Date: 2019-08-06
CROP INST SICHUAN PROVINCE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] At present, there are many anti-BPH genes, but only Bph14 has been cloned

Method used

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  • Rice brown planthopper resistance gene bph28 and its application
  • Rice brown planthopper resistance gene bph28 and its application
  • Rice brown planthopper resistance gene bph28 and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] (1) Cloning and determination of Bph28 candidate genes

[0042]Using Rathu Heenati / KDML105 by Jairin et al. The BC3F3 population locates the Bph3 segment in the 190kB region between the SSR markers RM19291 and RM8072 on chromosome 6. Downloaded the DNA sequence of Bph3 fine positioning segment from http: / / rice.plantbiology.msu.edu / , and predicted the gene in this segment by using the online gene prediction software Fgenes at http: / / linux1.softberry.com / berry.phtml , a total of more than 30 genes were predicted. According to the report of Jairin et al. (Jairin J., S. Teangdeerith, P. Leelagud, et al. Detection of brown planthopper resistance genes from different ricemapping populations in the same genomic location. Sci. Asia, 2007, 33:347-352), the For the predicted candidate genes, use Primer Premier6 to design exon primers for the remaining genes, PCR amplify Ptb33, Minghui 63, and TN1, and recover the amplified fragments (see "Primer Premier6.0 Manual" by PREMIER Bi...

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PUM

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Abstract

The invention discloses a rice anti-Nilaparvata-lugens gene Bph28 and application thereof. The rice anti-Nilaparvata-lugens gene Bph28 has a nucleotide sequence disclosed as SEQ.ID.NO.1. A biological informatic analysis and candidate gene clone sequencing method is utilized to separate the rice anti-Nilaparvata-lugens gene Bph28; and the Bph28 is subjected to genetic transformation, so that the insect-sensitive rice has anti-Nilaparvata-lugens phenotype, which proves the anti-Nilaparvata-lugens function of the gene.

Description

technical field [0001] The invention relates to the fields of molecular biology and agriculture, in particular to a rice brown planthopper resistance gene Bph28 and its application. Background technique [0002] Rice is one of the most important food crops in the world, and more than half of the world's population depends on it as a staple food. At the same time, as a model plant, the genome sequences of its two subspecies, japonica and indica, have been sequenced. The research on rice has entered the post-genome era, and the research on functional genes has become a hot spot in life science research. Therefore, analyzing the function of rice genes is of great significance to life science research and social and economic development. [0003] Ensuring food security is one of the major challenges facing the world. Pests and diseases are the main factors that threaten the high and stable yield of rice. Brown planthopper is one of the main pests and diseases of rice in my co...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/29C07K14/415C12N15/82A01H5/00A01H6/46
Inventor 任光俊任鄄胜高方远吴贤婷陆贤军
Owner CROP INST SICHUAN PROVINCE ACAD OF AGRI SCI
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