Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Rice brown planthopper resistance gene bph6 and its closely linked molecular markers

An anti-BPH and molecular marker technology, applied in the field of plant genetic engineering, can solve problems such as difficult operation, limited effect, and increased resistance of BPH, and achieve clear functions and good effects

Active Publication Date: 2018-02-23
WUHAN UNIV
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since the brown planthopper outbreaks mostly occur in the mature rice filling stage, when the rice plants are growing vigorously, it is very difficult to apply insecticides to the base of the rice plants.
In fact, due to the large-scale application of chemical insecticides year after year, the resistance of brown planthoppers has doubled, making the effect of pesticide control limited.
At the same time, the use of chemical pesticides to control brown planthoppers, on the one hand, increases the production cost of farmers, and on the other hand, chemical pesticides also cause environmental and ecological problems such as poisoning of non-target organisms, environmental and food pollution, etc.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Rice brown planthopper resistance gene bph6 and its closely linked molecular markers
  • Rice brown planthopper resistance gene bph6 and its closely linked molecular markers
  • Rice brown planthopper resistance gene bph6 and its closely linked molecular markers

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0067] Example 1 Positional cloning of Bph6 gene and development of linked molecular markers

[0068] 1. Preliminary positioning results of Bph6

[0069] A Bph6-containing F 2 Populations, 93-11 and Swarnalata were all obtained from the National Crop Germplasm Conservation Center of the Institute of Crop Science, Chinese Academy of Agricultural Sciences, and used the CTAB method (Murray MG&Thompson, 1980 Rapidisolation of high-molecular-weight plant DNA.Nucleic Acids Res 8:4321-4325) Extract parent and F 2 Genomic DNA of each individual plant in the population. each F 2 A single plant obtains the corresponding F 2:3 family lineage. To identify F 2 The N. lugens resistance phenotype of each individual plant in the localization population was investigated by using the seedling stage group method to investigate the resistance performance of each individual plant of the F2:3 family. 2:3 Family resistance level represents F 2 BPH-resistant phenotype of individual plants. T...

Embodiment 2

[0092] Example 2 Functional Verification and Application of Bph6 Gene

[0093] 1. Construction of genetic transformation vector

[0094] (1) Construction of Bph6 gene overexpression vector. The vector used is pCXUN (provided by Professor Wang Guoliang of Ohio State University in the United States), and the pCXUN vector is digested with XcmI, and the foreign fragment can be directly connected after adding A.

[0095] According to the results of RACE, PCR method was used to amplify the ORF directly, and after adding A, it was connected into the vector. After the sequence verification is correct, the obtained vector is the Bph6 gene overexpression vector, which is electrotransformed into Agrobacterium EHA105. Pick a single clone for expansion and culture, and after PCR verification, add an equal volume of 50% glycerol to mix, and store at -70°C for later use.

[0096] (2) Bph6 gene RNAi vector construction

[0097] The vector used is pCXUN (provided by Professor Wang Guoliang...

Embodiment 3

[0103] Example 3 Verification of molecular markers

[0104] 1. Materials and methods

[0105] 1.1 Materials: BPH-resistant parent Swarnalata (containing the brown planthopper-resistant gene Bph6), brown planthopper-susceptible rice variety Yangdao 6 (93-11) and F produced by crossing Swarnalata and Yangdao 6 23 family lineage.

[0106] Molecular marker primers: H and Y37, the nucleotide sequences of which are respectively shown in SEQ ID No.4-5.

[0107] 1.2 Method

[0108] Genomic DNA was extracted from rice samples by CTAB extraction. Sample DNA was amplified with primers H and Y37, respectively. 10 μl system. The 10 μl reaction system includes: 10×PCR buffer, 1.0 μl; 10 mM dNTPs, 0.1 μl; 10 μM primer, 0.4 μl; 5U / μl Taq DNA polymerase, 0.2 μl and 50ng DNA template. The amplification reaction was carried out on a Bioer PCR instrument: 94°C for 4min; 30 cycles of 94°C for 30s, 55°C for 30s, 72°C for 90s; 72°C for 5min. The amplified products of H were separated by 1% ag...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides the rice brown planthopper resistance gene Bph6 and its closely linked molecular markers. The nucleotide sequence of the rice brown planthopper resistance gene Bph6 is shown in SEQ ID No.1, and its cDNA sequence is shown in SEQ ID No.2. The Bph6 gene is located between molecular markers H and Y37, and is closely linked with them. Using molecular markers H and Y37 to screen rice containing gene Bph6 resistant to brown planthopper. Through genetic transformation and hybridization, the Bph6 gene is transferred into ordinary rice varieties, which can improve the resistance of rice to brown planthoppers, thereby reducing the damage caused by brown planthoppers, and achieving the purpose of increasing and stabilizing yields.

Description

technical field [0001] The invention belongs to the field of plant genetic engineering, in particular to a rice brown planthopper resistance gene Bph6 and its closely linked molecular markers. Background technique [0002] Rice is an important food crop, which is the staple food for more than half of the world's people. At the same time, since the fine genetic map and physical map of the rice genome have been completed, its transgenic technology is relatively easy, and it has collinearity with other grass crop genomes, so it is regarded as a model plant. With the completion of genome sequencing of various organisms including rice, human beings have entered the post-genome era. It has become the frontier field of life science to carry out functional genome research in an all-round way. Therefore, the study of rice functional genes is of great significance to socioeconomic development and biological research. [0003] Food security is a challenge faced by people all over th...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/29C07K14/415C12N15/82C12Q1/6895C12N15/11
CPCC07K14/415C12Q1/68C12N15/11C12N15/63C12N15/82A01H1/045
Inventor 何光存郭建平杜波邱永福陈荣智
Owner WUHAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com