Heterotrophic nitrification aerobic granule sludge, culture method thereof and use

A technology of aerobic granular sludge and heterotrophic nitrification, which is applied in the field of environmental microorganisms, can solve the problems of unstable functional properties of aerobic granular sludge, and achieve the effect of a wide range of substrate utilization and easy cultivation

Active Publication Date: 2008-11-19
CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
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  • Abstract
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Problems solved by technology

And since this process is completely a natural enrichment process of strains in the inoculated sludge, the functional characteristics of the aerobic granular sludge cultivated by the...

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  • Heterotrophic nitrification aerobic granule sludge, culture method thereof and use
  • Heterotrophic nitrification aerobic granule sludge, culture method thereof and use
  • Heterotrophic nitrification aerobic granule sludge, culture method thereof and use

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Embodiment 1

[0063] Embodiment 1: The cultivation of heterotrophic nitrifying aerobic granular sludge of the present invention

[0064] (1) Beef extract medium, sterilized at 121°C for 20 minutes.

[0065] (2) The strains Stenotrophomonas maltophilia strain DN 1.1 and Pseudomonas putida strain DN1.2 were inoculated into 500 mL of sterilized beef extract liquid medium, respectively, and cultured at 35° C. and 170 rpm for 24 hours. The cultured bacterial solution was centrifuged at 4000rpm for 5min, resuspended with a small amount of simulated wastewater, and then put into the reactor.

[0066] (3) Put 15g of activated carbon powder in a 500mL beaker, add 200mL of simulated wastewater, stir with a glass rod for 30min, and then transfer it to the reactor. The reactor is cylindrical, made of plexiglass, with a bottom diameter of 8cm, a height of 1.0m, an effective height of 80cm, and an effective volume of 4L. The 4 sampling ports are 60cm, 50cm, 40cm and 30cm away from the bottom in turn, a...

Embodiment 2

[0073] Example 2: Removal of ammonia nitrogen in simulated wastewater by heterotrophic nitrification and aerobic granular sludge of the present invention

[0074] Table 2 Removal of ammonia nitrogen from simulated wastewater by heterotrophic nitrification and aerobic granular sludge

[0075]

[0076] The ammonia nitrogen concentration of the simulated wastewater is 212mg / L, and the COD concentration is 4000mg / L. The reactor is cylindrical, made of plexiglass, with a bottom diameter of 8cm, a height of 1.0m, an effective height of 80cm, and an effective volume of 4L. The 4 sampling ports are 60cm, 50cm, 40cm and 30cm away from the bottom in turn, and the mud discharge port is 10cm away from the bottom. Configure ACO-012 electromagnetic air compressor and microporous aeration head, LZB glass rotor gas flowmeter. The reactor operates in SBR mode at low temperature (11-15°C), and the operation cycle is 12 hours, with water inflow for 5 minutes, aeration for 708 minutes, settl...

Embodiment 3

[0077] Embodiment 3: The treatment effect of heterotrophic nitrification aerobic granular sludge of the present invention on chicken farm wastewater

[0078] The chicken farm wastewater was taken from a chicken farm in Chengdu, the concentration of ammonia nitrogen was 755mg / L, and the concentration of COD was 8500mg / L. About 200 mL of heterotrophic nitrifying aerobic granular sludge was taken and washed 3 times with 500 mL of sterile saline. After the last washing, let it stand for 5 minutes, pour off the supernatant, take 150mL of this washed heterotrophic nitrification-aerobic granular sludge, add it to a 2L measuring cylinder, and add chicken farm wastewater to 1L. An aeration head is placed at the bottom of the measuring cylinder, the aeration rate is controlled at 80-90L / h, the reaction temperature is 11-15°C, and samples are taken regularly for measurement. The experimental results are shown in Table 3. As can be seen from Table 3, this heterotrophic nitrifying aerobi...

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Abstract

The invention belongs to the environmental microorganism field, in particular relating to heterotrophic nitrification aerobic granular sludge and a cultivation method and an application thereof. An inoculated strain of the aerobic granular sludge is stenotrophomonas maltophilia and pseudomonas putida. Activated carbon is adopted to prefix a functional strain culture, ordinary activated sludge is not inoculated, and the heterotrophic nitrification aerobic granular sludge can be cultivated. The aerobic granular sludge can effectively remove ammonia nitrogen, nitrite nitrogen, nitrate nitrogen and a mixture thereof in a water body, can also simultaneously remove CODCr in organic wastewater, is applicable to the treatment of high concentrated organic nitrogen-containing wastewater and inorganic nitrogen-containing wastewater, and does not produce the accumulation of nitrite and nitrate during the denitrification. The application of the aerobic granular sludge for wastewater treatment has simple process and steady denitrification effect.

Description

technical field [0001] The invention belongs to the field of environmental microorganisms, and in particular relates to a cultivation method and application of heterotrophic nitrification and aerobic granular sludge. Background technique [0002] Nitrogen-containing wastewater is a major problem in environmental pollution control. Biological denitrification is currently recognized as one of the most economical and effective methods for denitrification of wastewater. The characteristic of biological denitrification technology commonly used at present is that the denitrification process is converted from ammonia nitrogen into nitrogen gas by two different types of microorganisms, autotrophic nitrifying bacteria and heterotrophic denitrifying bacteria, through the coupled metabolism of nitrification and denitrification. [0003] Aerobic granular sludge technology is one of the new sewage biological treatment technologies that have developed rapidly in recent years. The unique...

Claims

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Application Information

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IPC IPC(8): C02F3/12C02F3/34C12N1/20C12R1/40
CPCY02W10/10
Inventor 黄钧牟丽娉
Owner CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
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