High-activity preservation and transportation solution for tumor tissues and preparation method thereof

A tumor tissue and transport fluid technology, applied in the biological field, can solve the problems of unsatisfactory tumor cell biological activity protection, unsatisfactory cell survival rate, etc. Effect

Active Publication Date: 2018-12-11
爱克精医(北京)生物医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The environmental requirements of tumor cells are different from those of normal cells. The disadvantage of tumor cell preservation solution is that the protection of the biological activity of tumor cells is not ideal, and the survival rate of preserved cells has not achieved satisfactory results.

Method used

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  • High-activity preservation and transportation solution for tumor tissues and preparation method thereof
  • High-activity preservation and transportation solution for tumor tissues and preparation method thereof
  • High-activity preservation and transportation solution for tumor tissues and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1-3

[0049] Embodiment 1-3 A kind of highly active tumor tissue preservation transport solution

[0050] Table 1

[0051]

[0052]

[0053] The method for preparing the tumor tissue preservation transport solution described in the above-mentioned embodiments 1-3 specifically includes the following steps:

[0054] (1) At first the DMEM culture fluid is divided into two parts equally;

[0055] (2) Take half of the DMEM culture solution and add antibiotics, amino acids and growth factors to obtain culture solution A;

[0056] (3) Take the other half of the DMEM culture solution and add sorbitol and insulin to obtain the culture solution B;

[0057] (4) Mix the culture solution A obtained in step (2) and the culture solution B obtained in step (3) evenly to obtain a highly active tumor tissue preservation transport solution.

[0058] The method for preserving tumor tissue in the transport solution for preserving tumor tissue with high activity comprises the following steps:

...

Embodiment 4-6

[0077] Example 4-6 A kind of highly active tumor tissue preservation transport solution

[0078] Table 4

[0079]

[0080]

[0081] Preparation method: Except for different ingredients, other operations and steps are the same as in Example 1-3.

[0082] Transportation method: same as Example 1-3.

[0083] Test Example

[0084] Divide the same volume of PBS buffer, DMEM cell culture fluid, and the tumor tissue preservation and transportation solution prepared according to the content disclosed in Examples 4-6 of the present invention into 5 groups of 100 tubes in each group as the preservation and transportation solution to store and transport tumor tissues, and the transportation time For 12 hours, the preserved tumor tissue was equalized.

[0085] The tumor tissues used were 5 tumor tissues from the same human breast cancer. After being transported to the destination and cultured for 2-3 weeks, the culture of the tumor cells was observed and their survival rates were...

Embodiment 7-9

[0096] Example 7-9 A kind of highly active tumor tissue preservation transport solution

[0097] Table 7

[0098]

[0099]

[0100] Preparation method: Except for different ingredients, other operations and steps are the same as in Example 1-3.

[0101] Transportation method: same as Example 1-3.

[0102] Test Example

[0103] Divide the same volume of PBS buffer solution, DMEM cell culture solution, and the tumor tissue preservation and transportation solution prepared according to the content disclosed in Examples 7-9 of the present invention into 5 groups of 100 tubes in each group as the preservation and transportation solution to store and transport tumor tissues, and the transportation time For 48 hours, the preserved tumor tissue was equalized.

[0104] The transported tumor tissues were 5 parts from the same human breast cancer tumor tissue. After being transported to the destination and cultured for 2-3 weeks, the culture of the tumor cells was observed and t...

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Abstract

The invention discloses a high-activity preservation and transportation solution for tumor tissues and a preparation method thereof. The high-activity preservation and transportation solution is prepared from the following components: a DMEM (Dulbecco's Modified Eagle Medium) culture solution and antibiotics; the preservation and transportation solution also contains amino acid, a growth factor, insulin and a certain amount of sorbitol, so that a tissue shape can keep relatively good; a prepared preservation solution has a very good anti-coagulation action and can be used for ensuring relatively high activity of the tumor tissues and prolonging the preservation time of the tumor tissues in vitro. According to the high-activity preservation and transportation solution, specific ingredientsand specific content of each component are controlled, so that the solution has a very good protection effect on the tumor tissues; the preservation and transportation solution can keep a morphological structure and bioactivity of the tumor tissues and prolong the preservation time of the tumor tissues in vitro, and can ensure the survival rate of primary culture of the tumor tissues better.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a tumor tissue highly active storage transport solution and a preparation method thereof. Background technique [0002] The storage solution must maintain the biological activity of the cells to meet its clinical application. Therefore, how to maintain the high activity of the cells becomes the key to treatment. Previously, people used normal saline or glucose as the protective solution, but due to lack of nutrients, differences in solution tension or isotonicity, etc., the cells experienced low viability, massive swelling and death during long-term storage or transportation. Subsequently, people continued to improve the composition of the storage solution and added KCl, sodium citrate, albumin, vitamins, etc. to form a storage solution. Although these preservation solutions maintain cell activity well for a long period of time, their components are complex and the preparation proce...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
CPCA01N1/021A01N1/0226
Inventor 马永贤
Owner 爱克精医(北京)生物医药科技有限公司
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