Real-time temperature-controlled miniature sample chamber

Abstract

The invention provides a real-time temperature-control micro sample room. The micro sample room comprises a temperature control device (A), a sample room body (B) and a temperature control instrument (C). The temperature control device (A) comprises a U-type supporting plate (1), a thermoelectric cooler (TEC) (2), a TEC metal heat-conduction piece (3), a thermocouple probe (4), a slide supporting plate (5) and a thermal insulation plate (6). The sample room body (B) comprises an upper cover slide (7), a sample room substrate (8) and a lower cover slide (9). The temperature control instrument (C) comprises a temperature control power supply, a feedback controller and a wire. The temperature of a sample can be controlled and adjusted in real time, the temperature control range is from 6 DEG C to 90 DEG C, the temperature response time is less than or equal to 3 min, and the temperature control accuracy ranges from minus 0.5 DEG C to 0.5 DEG C. No mechanical vibration is introduced to the sample room, no noise sources exist, the capacity is adjustable, the sample room can be full with at least 10 micro liters of solutions, the size is small and the sample room is especially suitable for being used in the situation that space of a microscope stage is very small due to a high-magnification-power objective lens.

Description

technical field [0001] The invention belongs to the technical field of micro-containers, and in particular relates to a micro-sample chamber capable of real-time temperature regulation. Background technique [0002] In microscopic observation experiments and micromanipulation experiments, ordinary sample chambers can only conduct experiments at room temperature because they have no temperature adjustment function. However, in practical applications, many biological, physical and chemical experiments need to maintain a specific temperature (not necessarily room temperature) during the experiment, or change the temperature as the experiment proceeds, so it is necessary to install a sample chamber with temperature control equipment. [0003] At present, the existing sample room control devices are generally large in size and often have noise sources such as fans, which cannot meet the ultra-low noise requirements required for high-magnification microscope objectives in a small ...

AI Technical Summary

Problems solved by technology

[0003] At present, the existing sample room control devices are generally large in size and often have noise sources such as fans, which cannot meet the ultra-low noise requirements required for high-magnification microscope objective lenses and high-precision measurements.

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