Application of Lycium barbarum polysaccharide in preparation of medicine for treating dry eye disease
A technology of Lycium barbarum polysaccharide and dry eye disease, applied in drug combination, sensory diseases and other directions, can solve the problems of slow onset, difficult to popularize and apply in people of different constitutions, etc., and achieve the effect of reducing lacrimal gland lesions and restoring the number of goblet cells.
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Embodiment 1
[0022] 1. Preparation of Lycium barbarum polysaccharide by decocting in water
[0023] (1) Take 10kg of wolfberry, dry it and crush it to a coarse powder, add 10 times and 8 times of water respectively, decoct twice for 1 hour each time; combine the decoctions from the two times, concentrate under reduced pressure, concentrate to 100L, and centrifuge on three legs Machine primary filtration, take supernatant filtrate 95L, and add 0.3% potassium sorbate to preserve.
[0024] (2) Use an ultrafiltration instrument, which is equipped with a roll-type ultrafiltration membrane, which allows the liquid to pass through the roll-type membrane in a laminar flow manner. Ultrafiltration pores with a certain molecular weight are distributed on the membrane. Molecular weight cut off and molecules smaller than this molecular weight are divided into two parts of liquid by ultrafiltration membrane. In this experiment, an ultrafiltration membrane with a molecular weight cut-off of 2000 was use...
Embodiment 2
[0034] 1. Preparation of wolfberry polysaccharide by microwave extraction
[0035] (1) Take 10kg of Chinese wolfberry, dry it and grind it into a coarse powder. Using pure water as solvent, the amount of water added is 10 times and 8 times the volume of the crude drug, respectively. Microwave extraction is performed twice with a 1KW-20KW microwave extractor, and 5 times are extracted respectively. For ~30 minutes, combine the two decoctions, concentrate under reduced pressure, and concentrate to 100L, the ratio of water extract to feed is 10:1, filter with a three-legged centrifuge, take 95L of supernatant filtrate, and add 0.3% sorbic acid Potassium antiseptic.
[0036] (2) Steps (2) to (8) are the same as in Example 1.
[0037] 2. Identification of polysaccharide content and molecular weight
[0038] Using the same method as in Example 1, the content of the Lycium barbarum polysaccharide obtained in this example was measured to be 97%, and the molecular weight of the Lyciu...
Embodiment 3
[0040] 1. Preparation of wolfberry polysaccharide by ultrasonic extraction
[0041](1) Take 10kg of wolfberry, dry it and crush it to a coarse powder, use pure water as the solvent, add water 10 times and 8 times the volume of the crude drug, extract twice with an ultrasonic extractor, extract for 5-30 minutes respectively, and combine The decoction was extracted twice, concentrated under reduced pressure, and concentrated to 100L. The ratio of water extract to feed was 10:1. It was first filtered by a three-legged centrifuge, and 95L of the supernatant filtrate was taken, and 0.3% potassium sorbate was added for preservation.
[0042] (2) Steps (2) to (8) are the same as in Example 1.
[0043] 2. Identification of polysaccharide content and molecular weight
[0044] Using the same method as in Example 1, the polysaccharide content of the freeze-dried powder Lycium barbarum obtained in this example was measured to be 96%, and the molecular weight was 2,000 Da to 2,000,000 Da....
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